Use of Molecular Diagnostic Techniques to Determinate the Epidemiology of Malaria Parasites in North Eastern Nigeria
The emergence of resistance to all antimalarial drugs in clinical use is now making it necessary to discover the markers responsible for the resistance. The principal aim of this research is the use of molecular diagnostic techniques to Determine the epidemiology of malaria parasites. Thirty blood samples were analyzed by microscopy and molecular techniques to monitor the relative efficiency in malaria diagnosis. Molecular analysis revealed 28 out of 30 samples as positive for malaria while Microscopic analysis revealed 27out of 30 samples as positive malaria parasite. The molecular analysis was particularly useful to unveil parasites presence in infections not detectable by blood smear analysis.
Keywords: Molecular Diagnostic Techniques, Epidemiology, Malaria Parasites
2. Flannery, E. L., Chatterjee, A. K., & Winzeler, E. A. Antimalarial Drug Discovery: Approaches and Progress towards New Medicines. Nature Reviews Microbiology, 2013; 849-863.
3. World Health Organisation. (2014, May 13). 10 Facts About Malaria. Retrieved from World Health Organisation: http://www.who.int/features/factfiles/malaria/en/
4. Tekwani, B. L., & Walker, L. A. Targetting the Hemozoin Synthesis Pathway for New Antimalarial Drug Discovery: Technologies for In vitro β-Hematin Formation Assay. Combinatorial Chemistry & High Throughput Screening, 2005; 8:63-79.
5. Noubiap, J. N. Shifting From Quinine to Artesunate as First-Line Treatment of Severe Malaria In Children And Adults: Saving More Lives. Journal of Infection and Public Health. 2014. doi:10.1016/j.jiph.2014.04.007
6. Hyde, G. The Molecular Epidemiology of Parasites. In G. Hyde, The Molecular Epidemiology of Trypanosomes and Leishmania (pp. 1-13). Landes Bioscience and Springer. 2011
7. Monis, P. T., Andrews , R. H., & Saint, C. P. Molecular biology techniques in parasite ecology. International journal of parasitology, 2002; 551-562.
8. Matthews, H., Idris, M. U., Khan, F., Read , M., & Nirmalan, N. Drug repositioning as a route to anti-malarial drug discovery: preliminary investigation of the in vitro anti-malarial efficacy of emetine dihydrochloride hydrate. Malaria Journal. 2013
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).