Use of Molecular Diagnostic Techniques to Determinate the Epidemiology of Malaria Parasites in North Eastern Nigeria

  • M.M. Lawan Department of Chemistry, Yobe State University Damaturu, Nigeria
  • and M.I. Shago Department of Chemistry, Yobe State University Damaturu, Nigeria

Abstract

The emergence of resistance to all antimalarial drugs in clinical use is now making it necessary to discover the markers responsible for the resistance. The principal aim of this research is the use of molecular diagnostic techniques to Determine the epidemiology of malaria parasites. Thirty blood samples were analyzed by microscopy and molecular techniques to monitor the relative efficiency in malaria diagnosis. Molecular analysis revealed 28 out of 30 samples as positive for malaria while Microscopic analysis revealed 27out of 30 samples as positive malaria parasite. The molecular analysis was particularly useful to unveil parasites presence in infections not detectable by blood smear analysis.


Keywords: Molecular Diagnostic Techniques, Epidemiology, Malaria Parasites

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References

1. Sabbatani, S., Fiorino, S., & Manfredi, R. The emerging of the fifth malaria parasite (Plasmodium knowlesi). A public health concern? Brazilian Journal of Infectious Diseases, 2010; 14(3):299-309.
2. Flannery, E. L., Chatterjee, A. K., & Winzeler, E. A. Antimalarial Drug Discovery: Approaches and Progress towards New Medicines. Nature Reviews Microbiology, 2013; 849-863.
3. World Health Organisation. (2014, May 13). 10 Facts About Malaria. Retrieved from World Health Organisation: http://www.who.int/features/factfiles/malaria/en/
4. Tekwani, B. L., & Walker, L. A. Targetting the Hemozoin Synthesis Pathway for New Antimalarial Drug Discovery: Technologies for In vitro β-Hematin Formation Assay. Combinatorial Chemistry & High Throughput Screening, 2005; 8:63-79.
5. Noubiap, J. N. Shifting From Quinine to Artesunate as First-Line Treatment of Severe Malaria In Children And Adults: Saving More Lives. Journal of Infection and Public Health. 2014. doi:10.1016/j.jiph.2014.04.007
6. Hyde, G. The Molecular Epidemiology of Parasites. In G. Hyde, The Molecular Epidemiology of Trypanosomes and Leishmania (pp. 1-13). Landes Bioscience and Springer. 2011
7. Monis, P. T., Andrews , R. H., & Saint, C. P. Molecular biology techniques in parasite ecology. International journal of parasitology, 2002; 551-562.
8. Matthews, H., Idris, M. U., Khan, F., Read , M., & Nirmalan, N. Drug repositioning as a route to anti-malarial drug discovery: preliminary investigation of the in vitro anti-malarial efficacy of emetine dihydrochloride hydrate. Malaria Journal. 2013
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Lawan M, Shago and M. Use of Molecular Diagnostic Techniques to Determinate the Epidemiology of Malaria Parasites in North Eastern Nigeria. JDDT [Internet]. 15Jan.2020 [cited 28Mar.2020];10(1):69-1. Available from: http://jddtonline.info/index.php/jddt/article/view/3843