Qualitative Phytochemical Screening, GCMS Studies and In-Vitro Anti-Oxidative Properties of Aqeuous Leaf Extract of Gnetum africanum
This study aimed at carrying out a qualitative phytochemical screening, GC-MS studies and in-vitro antioxidant properties of aqueous leaf extract of Gnetum africanum. The qualitative phytochemical screening of the aqueous leaf extract of Gnetum africanum was done using standard procedures and revealed the presence of terpenoids, saponins, tannins, steroids, flavonoids, alkaloids, cardiac glucosides and phenols. The GC-MS screening revealed the presence of 14 compounds, 6 out of the 14 compounds were most prominent. The compound with the highest percentage peak area was caffeine with peak area of 96.9%, followed by n-Hexadacanotic acid with peak area of 60.9%, 2-methoxy-4-vinylphenol with peak area of 55.9%, tetradacanoic acid with peak area of 50.3%, cyclopentaneundecanoic acid with peak area of 47.8% and 2-cyclo-penten-1-2-hydroxy with peak area of 43.6% respectively. In-vitro determination of antioxidant property of leaf extract of Gnetum africanum was done photometrically using 2,2-dyhenyl-l-picrylhydrazyl (DPPH) assay. The DPPH scavenging ability of the leaf extract (43.2, 60.5, 68.8, and 75.7) was statistically significant at p<0.05 when compared with the standard drug ascorbic acid (81.1, 82.6, 85.1, and 90.4) % at 10, 20, 30 and 40 mg/l. In conclusion, the leaf extract of Gnetum africanum is loaded with a host of important phytochemicals and has antioxidant properties which increase in potency with increase dose.
Keywords: Phytochemical Screening, GCMS Studies, Anti-Oxidant, Gnetum africanum
2. Cos, P., Arnold, J. V., Dirk, V. B. and Louis, M. (2006). Anti-infective potential of natural products: How to develop a stronger in vitro “proof-of-concept‟. Journal of Enthnopharmacology, 106: 290-302.
3. Clardy, J. and Walsh, C. Lessons from natural molecules. Nature, 2004; 432: 829 – 837.
4. Nostro A, Germano MP, Dangelo V, Marino A, Cannatelli MA. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett. Appl. Microbiol. 2000; 30:379- 384.
5. Edeoga, H.O., Okwu, D.E., Mbaebie, B.O. Phytochemical constituents of some Nigeria medicinal plants. Afr. J. Biotech, 2005; 4(7):685-688.
6. Ene-obong, H.N., Okudu, H.O., Asumugha, V.U. Nutrient and photochemical composition of two varieties of Monkey kola (Cola parchyarpa and Cola lepidota) an underutilized fruit. Food Chem, 2016; 193:154-9.
7. Ogbu JU, Umeokechukwu CE. Aspects of fruit biology of three wild edible monkey Kola species fruits (Cola spp: Malvaceae). Annual Research & Review in Biology; 2014; 4(12):2007-2014.
8. Dutta AC. Botany for degree students. 5th ed. Oxford: Oxford University Press; 2000, p. 37-42.
9. Okerulu IO, Onyema CT. Comparative Assessment of Phytochemicals, Proximate and Elemental Composition of Gnetumafricanum (Okazi) Leaves. American Journal of Analytical Chemistry; 2015; 6:604 – 609.
10. Eyo E, Abel U. Chemical composition and amino acid content of Gnetumafricanum leaves. Nig J NutrSci 1983; 4:52-57.
11. Burkill HM. Useful Plants of Tropical Africa. Vol. 2 families E-I. Royal Botanical Gardens, Kew. 1994; Pp 648.
12. Dike, M.C. Proximate and Nutrient Compositions of Some Fruits, Seeds and Leaves of Some Plant Species at Umudike, Nigeria. ARPN-Journal of Agricultural and Biological Science, 2010; 5:7-16
13. Ekop, A.S. and Eddy, N.O. Comparative Studies of the Level of Toxicants in the Seed of Indian Almond (Ter- minaliacatappa) and African Walnut (Coulaedulis). Chem. Class J., 2005; 2:74-76.
14. Ali F, Assanta MA & Robert C. Gnetum africanum: A wild food plant from the African forest with many nutritional and medicinal purposes. Journal of Medicinal Foods, 2011; 14(11):1289 – 1297
15. Mailoundama, F. Nutritional and Socio-Economic Value in Central African Forest. In: Itladikom, et al., Eds., Tropical Forest People and Food: Bio-Cultural Interactions and Applications to Development, Parthenon Publishing Group, Carnforth. 1993
16. Okafor JC. Conservation and use of traditional vegetables from woody forest species in Southeastern Nigeria. In: Guarino L (Ed). Proceedings of the IPGRI international workshop on genetic resources of traditional vegetables in Africa: Conservation and use 1995 Conference. Kenya: ICRAF. 1997; Pp 31-38.
17. Iwu MM. Handbook of African Medicinal Plants, second ed. Taylor and Francis Group, Florida. 2010; Pp 224-226.
18. Eleazu CO, Eleazu KC. Health promoting compounds and in vitro antioxidant activity of raw and decoctions of GnetumaficanumWelw. Asian Pac J Trop Dis 2013; 3(6):472-479.
19. Boxin OU, Dejian H, Maureen AF, Elizabeth KD. Analysis of antioxidant activities of common vegetables employing Oxygen Radical Absorbance Capacity (ORAC) and Ferric Reducing Antioxidant Power (FRAP) Assay: A comparative study. J. Agric. Food Chem. 2002; 5:223 – 338.
20. Senthil N, Manoharan S. Lipid peroxidation and antioxidants status in patients with papillary thyroid carcinoma in India. Asia Pac J ClinNutr; 2004; 13(4):391 – 395.
21. Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants, and the degenerative diseases of aging. Proceedings National Acad Sci. 1993 Sep 1; 90(17):7915-22.
22. Halliwell B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? 1994; 344(8924):721 – 724.
23. Yadav, A., Kumari, R., Yadav, A., Mishra, J.P., Srivatva, S. and Prabha, S. Antioxidants and its functions in human body-A Review. Res. Environ. Life Sci., 2016; 9(11):1328-1331.
24. Hearther, K. V. and Talcott, S. Phytochemicals in plant-based foods could help battle obesity, disease. Journal of Human Nutrition and Dietetics, October, 2009. University of Florida. 2009.
25. Dembinska-Kiec, A., Mykkanen, O., Kiec-Wilk, B. and Mykkanene, H. Antioxidants phytochemicals against Type 2 diabetes. British J. Nutri, 2008; 99: 109-117.
26. Tijjani MA, Abduraham FI, Buba SW, Mala GI, Akan JC, Aji BM, Abdullahi AS. Chemical and Proximate Content or Methanolic leaf extract of Pilistigma thonningii schum Camel foot. J. Chem. Pharm. Res. 2012; 4(5):2409-2414.
27. Willis, L.M., Shukitt-Hale, B. and Joseph, J.A. Recent advances in berry supplementation and age-related cognitive decline. Current opinion in clinical nutrition and metabolic care, 2009; 12: 91-94.
28. Trease, G. E. and Evans, W. C. Pharmacognisy.11th edn.BrailliarTiridel Can. Macmillian publishers. 1989.
29. Mensor, LL, Fabio, SM, Gilda, GL, Alexandre, SR., Tereza, CD, Cintia, SC Suzana, GL. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother. Res.; 2001; 15:127-130.
30. Oteng-Gang, K. and Mbachu, J.I. Changes in the Ascorbic Acid Content of Some Tropical Leafy Vegetables during Traditional Cooing and Local Processing. Journal of Food Chemistry, 1990; 23:9-17.
31. Owolabi OJ, Amaechina FC, Okoro M. Effect of ethanol leaf extract of Newbouldialaevis on blood glucose level of diabetic rats. Trop. J. Pharm. Res. 2011; 10(3):249-254.
32. Egba SI, Uzoegwu PN, Emmanuel TN, Joshua PE. Anti- sickling potential of the ethanol seed extract of Vignaunguiculata and Vigna subterranean. Int. J. Adv. Pharm. Nanotechnol. 2012; 2(3):178-181.
33. Kuzuyama T, Seto H. Diversity in the biosynthesis of Isoprene units. Nat. Prod. Rep. 2003; 20(2):171-183.
34. Tijjani MA, Abduraham FI, Buba SW, Mala GI, Akan JC, Aji BM, Abdullahi AS. Chemical and Proximate Content or Methanolic leaf extract of Pilistigma thonningii schum Camel foot. J. Chem. Pharm. Res. 2012; 4(5):2409-2414
35. Mensah JK, Irenyen JO, Okhiure MO. Nutritional Phytochemical and Antimicrobial Properties of two wild Aromatic Vegetables from Edo State. J. Nat. Prod. Plants Res. 2013; 3(21):8-14.
36. Asquith TN, Butler LG. Interaction of condensed tannin with proteins. Phytochemistry 1986; 2(7):1591-1593.
37. Okwu DE. Evaluation of the chemical composition of indigenous spices and flavouriing agents. Global J. Pure Appl. Sci. 2001; 7:455-459.
38. Poli G, Cottalaso D, Prontazo M, Chairpotto E. Lipid peroxidation and covalent binding in the early functional impairment of liver Golgi Apparatus by CCl4. Cell Biochem. Funct. 1990; 8:1-10.
39. Sakihama, Y., Cohen, M. F., Grace, S. C. and Yamasaki, H. Plant phenolic antioxidant and prooxidant activities: phenolics-induced oxidative damage mediated by metals in plants. Toxicology, 2002; 177:67-80.
40. Rice-Evans, C., Miller, N. and Paganga, G. Antioxidant properties of phenolic compounds. Trends Plant Sci. 1997; 2:152 – 159.
41. Nwankpa P, Chukwuemeka OG, Ugwuezumba PC, Ekweogu CN, Etteh CC, Emengaha FC, Egwurugwu JN, Ngwu EE. Assessment of Anti-Oxidative Potential of Ethanol Seed Extract of Cola lepidota in High Fat Fed Female Wistar Albino Rats. East African Scholars J Med Sci; 2018; 1(2):47 – 53.
42. Esterbauer, H., Eckl, P., Ortner, A. Possible mutagens derived from lipids and lipid precursors. Mutagen Research; 1990; 238:223 – 233.
43. Oktay, M. Gulcin, I. and Kufrevioglu, O. I. Determination of in vitro antioxidant activity of Fennel (Foeniculumvulgare) seed extracts. Lebensm. Wiss Technol. 2003; 36:363-371.
44. Essien, E. E., Peter, N. S. and Akpan, S. M. Chemical Composition and Antioxidant Property of Two Species of Monkey Kola (Cola rostrata and Cola lepidota K. Schum) Extracts. Euro. J. Med. Plants, 2015; 7(1):31-37.
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