Instrumental Characterization and Antibacterial Investigation of Silver Nanoparticles Synthesized From Garcinia Kola Leaf

  • Sunday Adewale AKINTELU Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
  • Aderonke Similoluwa FOLORUNSO Department of Chemistry, Louisiana State University, Louisiana, USA
  • Olabisi Theresa ADEMOSUN Department of Chemical science, Covenant University, Otta, Nigeria


The need to devise another method of synthesizing nanoparticles from sources that are eco-friendly, non-hazardous and cost effectiveness is of great importance in preventing environmental and health problems. The aim of this study was to evaluate the efficiency of Garcinia kola leaves as reducing and stabilizing agent for silver nanoparticles synthesis. The leaves of Garcinia kola obtained were authenticated, air dried, pulverized and extracted. The extract was mixed with aqueous solution of silver nitrate solution to form silver nanoparticles and were characterized using Ultra violet (UV) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), Energy-dispersive X-ray spectroscopy (EDX) and X-ray diffraction (XRD). The antibacterial investigation of the synthesized silver nanoparticle was carried out following the disk diffusion method. UV analysis revealed the silver surface plasmon band at 425.18 nm, The FTIR indicated -OH, -C=C- and alkane as the functional groups responsible for the stabilization of the silver nanoparticle formed. The morphological assessment from SEM and TEM analysis confirmed that the silver nanoparticle formed are spherical in shape with an average particle size of 28.80nm.The EDX analysis ascertained that the silver surface plasmon resonance at 2.8–3.2 keV was confirmed the reduction of silver ion (Ag+ to Ag0). The XRD study revealed the crystalline nature of the nanoparticles synthesized. The antibacterial investigation showed high inhibition against the growth of tested bacteria.  This study ascertained that the green synthesis of silver nanoparticle without the use of harmful solvent that are offensive to the environment is achievable. 

Keywords: Silver nanoparticles, Biosynthesis, Characterization, Antibacterial activity and Garcinia kola.


Download data is not yet available.


[1] Franci G, Falanga A, Galdiero S, Palomba L., Rai M Silver nanoparticles as potential antibacterial agents. Mol. 2015; 20:8856–8874
[2]Mallevre F, Fernandes TF, Aspray TJ. Pseudomonas putida biofilm dynamics following a single pulse of silver nanoparticles. Chemosph. 2016; 153:356–364
[3] Guzmán MG, Dille J, Godet S. Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity. Int J Chem Biomol Eng. 2009; 2(3):104–11.
[4] Song KC, Lee Sm, Park TS Lee BS. Preparation of colloidal silver nanoparticles by chemical reduction method. Korean J Chem Eng. 2009; 26 (1):153–5.
[5] Rashid MU, Bhuiyan MKH, Quayum ME. Synthesis of silver nano particles (Ag-NPs) and their uses for quantitative analysis of vitamin C tablets. Dhaka Univ J Pharm Sci. 2013; 12(1):29–33.
[6] Qin Y, Ji X, Jing J, Liu H, Wu H, Yang W. Size control over spherical silver nanoparticles by ascorbic acid reduction. Colloids Surf A Physicochem Eng Asp. 2010; 372 (1–3):172–6.
[7] Kowshik M, Ashtaputre S, Kharrazi S, Vogel W, Urban J Kulkarni, S.K, Paknikar K.M. Extracellular synthesis of silver nanoparticles by a silver-tolerant yeast strain MKY3. Nanotech. 2002; 14 (1):95.
[8] Natarajan K, Selvaraj S, Murty VR. Microbial production of silver nanoparticles. Dig J Nanomater Biostruct. 2010; 5(1):135–40.
[9] Prakash P, Gnanaprakasam P. Emmanuel R. Saravanan M. Green synthesis of silver nanoparticles from leaf extract of Mimusops elengi, Linn for enhanced antibacterial activity against multi drug resistant clinical isolates. Colloids Surf B: Biointer. 2013; 108:255–9
[10] Antonio Z, Annalaura I, Stefano N and Roberto L Green Synthesis of Silver Nanoparticles Using Bilberry and Red Currant Waste Extracts. Proc. 2019; 7, 193; 1-12
[11] Abudalo MA, Al-Mheidat IR, Al-Shurafat AW, Grinham C, Oyanedel-Craver V. Synthesis of silver nanoparticles using a modified Tollens’ method in conjunction with phytochemicals and assessment of their antimicrobial activity. Peer J. 2019; 1-22
[12] Balavijayalakshmi J., Ramalakshmi V 2019. Carica papaya peel mediated synthesis of silver nanoparticles and its antibacterial activity against human pathogens. J Appl Res Techn 2017; 413–422
[13] Folorunso A, Akintelu S , Oyebamiji AK, Ajayi S , Abiola B , Abdusalam I , Morakinyo A, Biosynthesis, characterization and antimicrobial Activity of gold nanoparticles from leaf extracts of Annona muricata. J Nanostru. Chem. 2019;111-117
[14] Akintelu SA, Folorunso AS. Characterization and Antimicrobial Investigation of Synthesized Silver Nanoparticles from Annona muricata Leaf Extracts. HSOA Journal of Nanotechnology: Nanomedicine & Nanobiotechnology 2019; 6: 1-5.
[15] Jerushka S M, Suresh B N K, Karen P, Sershen and Patrick G. Adv Nat Scis: Nanosci Nanotech. 2018; 1-9
[16] Akhil R, Jyoti R and Mira D. Green synthesis of silver nanoparticles from Tectona grandis seeds extract: characterization and mechanism of antimicrobial action on different microorganisms. J Anal Sci Techn. 2019; 1-10
[17] El-Saadony MT, El-Wafai NA, El-Fattah HIA, Mahgoub SA . Biosynthesis, optimization and characterization of silver nanoparticles using a soil isolate of Bacillus pseudomycoides MT32 and their antifungal activity against some pathogenic fungi. Adv. Anim. Vet. Sci. 2019; 7(4): 238-249.
[18] Saba P, Maryam G, Saeid B. Green synthesis of silver nanoparticles using the plant extract of Salvia spinosa grown in vitro and their antibacterial activity assessment. J Nanostru Chem 2019; 9:1–9
[19] Oyebamiji A K, Akintelu S A, Folorunso A S., Abiola B E., Ajayi Samuel O., Abdusalam I O., and Morakinyo A E. Computational And Experimental Studies On Antimicrobial Activity Of The Bark Of Annona Muricata Against Some Selected Human Pathogenic Bacteria And Fungi. Int J Mod Chem 2019; 11(1): 9-27
[20] Yohannes KE, Ebrahim AS, Tekleab T T, and Gedefaw GA. Antibacterial Potential of Aloe weloensis (Aloeacea) Leaf Latex against Gram-Positive and Gram-Negative Bacteria Strains. Int J Microbio. 2019;1-4
[21] Folorunso A.S, Oyebamiji A.K, Erazua E.A and Akintelu S.A. The Exploration of Antifungal Activity of Garcinia kola Seed as Novel Antifungal Agent. Int. J Trad Nat Med, 2019; 9(1): 41-49
[24] Akintelu S.A, Abiola B.E, Ajayi S.O and Olabemiwo O.M Quantification and Preliminary Estimation of Toxic Effects of Polycyclic Aromatic Hydrocarbon in Some Antimalarial Herbal Drugs in Southwest Nigeria. Bull Pharm Res. 2018; 8(1):152.
[23] Akintelu S.A, Abiola B.E, Ajayi S.O, Olabemiwo O.M (2019) Preliminary Study of the Effects of some Herbal Drugs on the Heamatological and Biochemical Parameter of Winster Albino Rats. Int J Sci. 2019; 8(10) 26-31
38 Views | 60 Downloads
How to Cite
AKINTELU, S. A., FOLORUNSO, A. S., & ADEMOSUN, O. T. (2019). Instrumental Characterization and Antibacterial Investigation of Silver Nanoparticles Synthesized From Garcinia Kola Leaf. Journal of Drug Delivery and Therapeutics, 9(6-s), 58-64.