Antioxidant and Anti-Inflammatory Activities of Caralluma Acutangula (Decne.) N.E.Br Extracts, a Medicinal Plant from Burkina Faso
Plants have always played an important role in health care in Africa. The stress, a situation of imbalance between oxidizing and antioxidant systems in favor of the prooxidants is responsible for the installation of several pathologies such as cancers, cardiovascular diseases, diabetes ... The objective of this study was to highlight the presence Secondary metabolites in C. acutangula extract and determine its antioxidant and anti-inflammatory potential.
For the determination of the acute toxicity of the extract, a dose of 2000 mg / kg was administered to the NMRI Mouse. The methods of screening were used to detect secondary metabolites like tannins, steroids and terpen, flavonoids, coumarins. The antioxidant capacity was evaluated in vitro by determining the ability of the extract to inhibit lipid peroxidation, hydrogen peroxide, degradation of deoxyribose. The anti-inflammatory potential was evaluated on lipoxygenase and xanthine oxidase. Acute toxicity evaluated in NMRI mice showed that the ethanolic extract of C. acutangula show no toxicity. Tannins, steroids and terpen, flavonoids, coumarins have been detected in the extracts. C. acutangula showed good activity with an inhibition of 50.71 ± 2.51% at 100 μg / ml on lipid peroxidation, of 66.105 ± 1.26% on deoxyribose degradation and 8.625 ± 1.09% on hydrogen peroxide. It showed good activity on xanthine oxidase with an 81.5 ± 5.5% inhibition. For the effect on lipoxygenase it gave an inhibition of the enzyme at 43.11 ± 3.4%. This potential could be used in the fight against inflammatory diseases and that due to oxidative stress.
Keywords: antioxidant, anti-inflammatory, oxidative stress, lipid peroxidation
2. Meriem B, Nesrine Z, L’activité biologique de l’extrait butanolique de la plante Genista sp. : Effet sur L’hépatotoxicité induite par la Doxorubicine Mémoire Master, 2015 ; 70P
3. Habbout A, Etude des conséquences métaboliques, oxydatives et cardiovasculaires de la suralimentation postnatale chez le rat et la souris. Thèse doctorat, 2012 ; 1-163.
4. Salvayre R, Auge N, Nègre-Salvayre A. Rôle de l’oxydation dans la genèse et la rogression de l’athérosclérose. L’athérosclérose : Physiopathologie, Diagnostics, Thérapeutiques., Eds. Masson: Paris, 2003 ; 14, P. 269-29.
5. Cillard J, Cillard P, Mécanismes de la peroxydation lipidique et des anti-oxydations. OCL Oleagineux- Corps gras. Lipides, 2006, 13(1) : 24-30.
6. Nijveldt RJ, Nood EV, Van Hoorn DEC, Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr, 2000 ; 74 (4) : 418-25.
7. Schmelzer GH, Gurib-Fakim A. “Medicinal plants 2” PROTA, 2013
8. Wilson K, Bernard W, Hillary R, Gabriel K, A study of the medicinal plants used by the Marakwet Community in Kenya, Journal of Ethnobiology and Ethnomedicine, 2014 ; 10:24
9. Ligne directrice 423 de l’OCDE pour les essais de produits chimiques, Toxicité orale aiguë - Méthode par classe de toxicité aiguë, 2001; 1-14.
10. Ciulei I. Pratical manuals on the industrial utilization of medicinal and aromatic plants: Methodology for analysis of vegetable drugs. Ministry of Chemical Industy, Bucharest, 1982; 1-67
11. Chang CL, Lin CS, Lai GH, Phytochemical Characteristics, Free Radical Scavenging Activities, and Neuroprotection of Five Medicinal Plant Extract. Evidence-Based Complementary and Alternative Medicine, 2012 ; 1-8.
12. Perjési P, Rozmer Z, Kinetic analysis of some chalcones and synthetic chalcone analogues on the fenton-reaction initiated deoxyribose degradation assay. The Open Medicinal Chemistry Journal, 2011 ; 5, 61–7
13. Ohkawa H, Ohishi N, Yagi K, Assay for lipid peroxidation in animal tissues by thiobarbituric acid reaction. Ann. Biochem, 1979 ; 95, 351–35
14. Mohan SC, Balamurugan V, Salini ST, Rekha R, Metal ion chelating activity and hydrogen peroxide scavenging activity of medicinal plant Kalanchoe pinnata. Journal of Chemical and Pharmaceutical Research, 2012 ; 4(1) : 197–202.
15. Malterud KE, Rydland KM, Inhibitors of 15-lipoxygenase from orange peel.Journal of Agricultural and Food Chemistry, 2000 ; 48, 5576-5580
16. Filha FZSIF, Vitolo LG, Fietto JAL, Saude-Guimaraes D.A. Xanthine oxidase inhibitory activity of Lychnophora species from Brazil ("Arnica"). J. Ethnopharmacol., 2006 ; 107, 79-82.
17. Chin-Lin H, Gow-Chin Y, Effects of Flavonoids and Phenolic Acids on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes. J. Agric. Food Chem., 2007 ; 55 (21) : 8P.
18. Kostova I, Bhatia S, Grigorov P, Balkansky S, Parmar VS, Prasad AK, Saso L, Coumarins as antioxidants. Curr Med Chem., 2011 ; 18(25) : 3929-51
19. Araùjo TAS, Alancar NL, Amorim ELC, Albuqerque CFTL, A new approach to study medicinal plants with tannins and flavonoids contents from local knowledge.Journal of Ethnopharmacology, 2008 ; 120, 72-80.
20. Jassim SAA, Naji MA, Novel antiviral agents: a medicinal plant perspective.Journal of Applied Microbiology, 2003 ; 95, 412-427.
21. Hong JH, Kim MJ, Park MR, Kwag OG, Lee IS, Byun BH, Lee SC, Lee KB, Rhee SJ, Effects of vitamin E on oxidative stress and membrane fluidity in brain of streptozotocininduced diabetic rats. Clin Chim Acta.2004 ; 340(1-2) : 107-15.
22. Roginsky V, Tashlitsky V, Skulachev V, (2009). Chain‐breaking antioxidant activity of reduced forms of mitochondria‐targeted quinones, a novel type of genoprotectors . aging, 2009 ; 1( 5)
23. Visconti R, Grieco D, New insights on oxidative stress in cancer. Curr. Opin. Drug Discov. Devel. 2009 ; 12, 240–245.
24. Dandona P, Aljada A, Chaudhuri A, Mohanty P, Garg R, Metabolic syndrome : A comprehensive perspective based on interactions between obesity, diabetes, and inflammation. Circulation, 2005 ; 111, 1448–1454.
25. Serafini M, Peluso I, Raguzzini A, Flavonoids as anti-inflammatory agents. Proc Nutr Soc. 2010 ; 69(3) :273-8.
26. GAUTHIER C, Glycosidation de triterpènes pentacycliques de type lupane et évaluation in vitro de leur potentiel anticancéreux. These de doctorat, Université de Quebec, 231P.
27. Bruneton J. Pharmacognosie Phytochimie Plantes médicinales. 2ème Ed. Tec et Doc. Lavoisier, Paris, 1993, 915p
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).