Synthesis, Characterization, in vivo acute toxicity and superoxide anion scavenging evaluation of new isatin-hydrazone

  • Kamel Mokhnache Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria
  • Ahlem Karbab Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria
  • Soraya Madoui Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria
  • Hanane Khither Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria
  • EL-Khamsa Soltani
  • Noureddine Charef Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Abstract

A new isatin-hydrazone (I); N'-[(E)-(5-bromo-1H- indol-3-yl) methylidene] pyridine-4-carbohydrazide  was prepared from the condensation reaction of 5-bromo-1H-indole-3-carbaldehyde and the anti-tubercular drug; isoniazid, in the presence of acetic acid. The obtained hydrazone was identified and characterized by physico-chemical techniques such as melting point, IR, NMR, and mass spectroscopy. In addition, the acute toxicity was evaluated using mice. The antioxidant of I was evaluated against superoxide anion radical.  Our biological results indicate low toxicity of I at the high dose of 1000 mg/kg, and high superoxide anion scavenging effect with inhibition percentage of 82.57 % and IC50 138.78 µg/mL.


Keyword: hydrazone, toxicity, antioxidant, superoxide anion

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Author Biographies

Kamel Mokhnache, Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Ahlem Karbab, Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Soraya Madoui, Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Hanane Khither, Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

EL-Khamsa Soltani

Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Noureddine Charef, Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

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How to Cite
Mokhnache, K., Karbab, A., Madoui, S., Khither, H., Soltani, E.-K., & Charef, N. (2019). Synthesis, Characterization, in vivo acute toxicity and superoxide anion scavenging evaluation of new isatin-hydrazone. Journal of Drug Delivery and Therapeutics, 9(6), 12-16. https://doi.org/10.22270/jddt.v9i6.3656

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