Synthesis, Characterization, in vivo acute toxicity and superoxide anion scavenging evaluation of new isatin-hydrazone
A new isatin-hydrazone (I); N'-[(E)-(5-bromo-1H- indol-3-yl) methylidene] pyridine-4-carbohydrazide was prepared from the condensation reaction of 5-bromo-1H-indole-3-carbaldehyde and the anti-tubercular drug; isoniazid, in the presence of acetic acid. The obtained hydrazone was identified and characterized by physico-chemical techniques such as melting point, IR, NMR, and mass spectroscopy. In addition, the acute toxicity was evaluated using mice. The antioxidant of I was evaluated against superoxide anion radical. Our biological results indicate low toxicity of I at the high dose of 1000 mg/kg, and high superoxide anion scavenging effect with inhibition percentage of 82.57 % and IC50 138.78 µg/mL.
Keyword: hydrazone, toxicity, antioxidant, superoxide anion
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