Evaluation of isoniazid-oxidative reactions in mice model

in vivo oxidative stress of isoniazid

  • Kamel Mokhnache Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria
  • Ahlem Karbab Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria
  • Soraya Madoui Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria
  • Hanane Khither Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria
  • El-Khamsa Soltani Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria
  • Noureddine Charef Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Abstract

In this study the anti-tubercular drug; isoniazid (INH) was investigated for their adverse effect; the oxidative stress. This effect was evaluated by using mice model, at the dose of 151 mg/kg.  We found that oxidative stress induced by INH is associated with lipid peroxidation expressed by the increase in the level of MDA from 76.9 ± 1.74 to 79.61 ± 2.67 nmol/g tissue. The oxidative stress of INH is accompanied by a decrease in reduced GSH level (from 79.9 ± 12 μmol / mg  to 68.48 ± 4.28 μmol / mg compared to of the control group). After treatment with INH at 151 mg/kg, a decrease in CAT activities occurred compared to control (2.53 ± 0.39 U/mg Pr vs 5.07 ± 0.73 U/mg Pr).


Keywords: isoniazid, oxidative stress, MDA, GSH, CAT

Downloads

Download data is not yet available.

Author Biographies

Kamel Mokhnache, Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Ahlem Karbab, Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Soraya Madoui, Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Hanane Khither, Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

El-Khamsa Soltani, Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Noureddine Charef, Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Applied Biochemistry, University Ferhat Abbas Setif 1, 19000, Algeria

References

[1] Koul A, Arnoult E, Lounis N, Guillemont J, Andries K, The challenge of new drug discovery for tuberculosis.Nature. 469 (2011) 483–490.
[2] Tafazoli S, Mashregi M, O'Brien PJ, Role of hydrazine in isoniazid-induced hepatotoxicity in a hepatocyte inflammation model, Toxicology and applied pharmacology. 229 (2008) 94–101.
[3] Rickman KA, Swancutt KL, Mezyk SP, Kiddle JJ, Isoniazid: Radical-induced oxidation and reduction chemistry. Bioorganic & medicinal chemistry letters.23 (2013) 3096–3100.
[4] Timmins GS, Master S, Rusnak F, Deretic V, Nitric Oxide Generated from Isoniazid Activation by KatG: Source of Nitric Oxide and Activity against Mycobacterium tuberculosis. Antimicrobial agents and chemotherapy. 48 (2004) 3006–3009.
[5] Georgieva N, Gadjeva V, Dimitrova D, Study on the influence of isoniazid alone or combined with new synthetized isoniazid structural analogues upon catalase activity, Bulgarian journal of veterinary medicine. 7 (2004) 9–16.
[6] Aouachria S, Boumerfeg S, Benslama A, Benbacha F, Guemmez T, Khennouf S, Arrar L, Baghiani A. Acute, sub-acute toxicity and antioxidant activities (in vitro and in vivo) of Reichardia picroide crude extract. Journal of Ethnopharmacology. 208 (2017) 105–116
[7] Suzen S, Cihaner SS, Coban T, Synthesis and Comparison of Antioxidant Properties of Indole-Based Melatonin Analogue Indole Amino Acid Derivatives, Chemical biology & drug design. 79 (2012) 76–83.
[8] Suresh R, Naik N, Vandana S, Panda M. Hepatoprotective effect of Ginkgo select Phytosome in rifampicin induced liver injurym in rats: Evidence of antioxidant activity. Fitoterapia. 79 (2008) 439–445.
[9] Mates JM, Perez-Gomez C, Nunenez de Castro I, Antioxidants enzymes and human diseases, Clinical Biochemistry. 32 (1999) 595–603.
Statistics
49 Views | 72 Downloads
How to Cite
Mokhnache, K., Karbab, A., Madoui, S., Khither, H., Soltani, E.-K., & Charef, N. (2019). Evaluation of isoniazid-oxidative reactions in mice model. Journal of Drug Delivery and Therapeutics, 9(6), 36-37. https://doi.org/10.22270/jddt.v9i6.3654

Most read articles by the same author(s)