Phytochemical Screening, Anti-Oxidant Potential and Anti- Ulcer Activity of Leucas diffusa Plant Extract
Abstract
Leucas diffusa (lamiaceae) a branched herb collected from the Guntur region, A.P. India. Phytochemical studies of Leucas diffusa, methanolic fraction subjected to column chromatography and estimation of total flavanoid content and to evaluate antioxidant potential and anti-ulcer activity of methanolic extract of Leucas diffusa. The plant powder was subjected to continuous hot extraction in Soxhlet Apparatus & extracted successively with n-hexane, ethyl acetate and methanol solvents. The extracts prepared were tested for the type of chemical constituents present by known qualitative tests. Total flavanoid content of methanolic and ethylacetate extract of Leucas diffusa were done. In vitro Antioxidant activity of methanolic extract was evaluated using hydroxyl radical, reducing power & hydrogen peroxide scavenging abilities. Anti-ulcer activitiy of methanolic extract was evaluated through pylorus ligation method and ethanol induced ulcer models. Statistical analysis was performed by one-way analysis of variance (ANOVA) followed by the tukey’s test for multiple comparisons. Our findings suggest that methanolic extract of whole plant Leucas diffusa have got profound antioxidant effect and showed significant anti-ulcer activity at higher dose.
Keywords: Leucas diffusa; Antioxidant; Flavanoid; anti-ulcer activity.
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