Synthesis, Characterization and Anti-Microbial Activity of Indole Derivatives

  • Anil Kumar Gupta
  • Manoj Sharma

Abstract

Indoles are probably the most widely distributed heterocyclic compound in nature. Tryptophan and essential amino acid as such is constituent of most proteins. Starting materials were identified by physical, chromatographic and spectral analysis. For substituted isonitroso acetanilide 3.6 gm (0.05M) of chloral hydrate and 48 ml of purified water was taken in it. Then 44 gm of crystallized anhydrous sodium sulfate was added in it and a solution of substituted aniline (0.05 M) in 12 ml of water with 1.7 ml (0.052M) of concentrated hydrochloric acid was added to dissolve the amine, and finally, a solution of 4.5gm (0.158M) of hydroxylamine hydrochloride in 20 ml of water.for preparation of  substituted isatin from substituted isonitroso acetanilide 32.5 ml of concentrated sulfuric acid was warmed upto 50°C in a 100 ml round bottom flask with continuous stirring, and 7.5 gram of (0.046 M) of dry substituted isonitroso acetanilide was added to such a rate that to keep the temperature 60-70 but not higher. All synthesized final products were screened for in vitro antibacterial activity [ 13-19, 20-22] against four bacterial strains, namely Staphylococcus aureus (MTCC 96), Staphylococcus pyogenus, Pseudomonas aeruginosa (MTCC 1688), Escherichia coli (MTCC 443) and two fungal strains, namely Candida albicans (MTCC 227) and Aspergilla niger (MTCC 282). in-vitro antimicrobial activity with the zone Inhibition in mm 24±2 and activity index 0.89, against Staphylococcus aureus, 22±2 and 0.85 against Staphylococcus pyogenes, 26±2 and 0.96 against Pseudomonas aeruginos, 25±3 and 0.86 against Escherichia coli and two fungal strains shown 26±4 and 0.81 against Candida albicans and 14±2 & 0.88 against Aspergilla niger respectively.


Keywords: Indole, Isatin, Antibacterial activity, Staphylococcus aureus.

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Author Biographies

Anil Kumar Gupta

Research Scholar, Institute of Pharmaceutical Science & Research Center, Bhagwant University, Ajmer, Rajasthan (India)

Manoj Sharma

Institute of Pharmaceutical Science & Research Center, Bhagwant University, Ajmer, Rajasthan (India)

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1.
Gupta AK, Sharma M. Synthesis, Characterization and Anti-Microbial Activity of Indole Derivatives. JDDT [Internet]. 25Aug.2019 [cited 18Jan.2021];9(4-s):918-25. Available from: http://jddtonline.info/index.php/jddt/article/view/3639