In Vitro Antidiabetic Effect of Neohesperidin

  • Durgeshnandani Sinha
  • Trilochan Satapathy
  • Parag Jain
  • Jhakeshwar Prasad Chandel CSVTU
  • Divya Sahu
  • Bhavna Sahu
  • Abhishek Verma
  • Shalini Singh
  • Khushboo Verma
  • Rahul Rathore


Objective: The present study was performed to determine in vitro antidiabetic effect of neohesperidin. To evaluate inhibitory effect of neohesperidin on α-amylase and α-glucosidase diabetes causing enzyme.

Methods and Materials: Invitro carbohydrate metabolizing enzyme based inhibitory methods were used to determine antidiabetic effect of neohesperidin. Alpha (α)-amylase inhibitory assay was performed using different sources i.e. wheat alpha (α)-amylase enzyme, salivary alpha (α)-amylase and fungal alpha (α)-amylase assay. Alpha (α)-glucosidase inhibitory assay was performed using alpha (α)-glucosidase (B. stearothermophil), alpha (α)-glucosidase rat intestine and alpha (α)-glucosidase from baker’s yeast. Sucrase inhibitory assay from rat small intestine.

Result: Neohesperidin possess a potent anti-diabetic by significantly inhibiting alpha amylase activity.

Conclusion: It was concluded that enzyme inhibitory activity of neohesperidin shown a significantly higher inhibitory activity on alpha-amylase in comparision to alpha-glucosidase & Sucrase enzymes.

Keywords: Neohespiridin, acarbose, alpha-amylase, alpha-glucosidase


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Sinha D, Satapathy T, Jain P, Chandel J, Sahu D, Sahu B, Verma A, Singh S, Verma K, Rathore R. In Vitro Antidiabetic Effect of Neohesperidin. JDDT [Internet]. 15Nov.2019 [cited 27Nov.2021];9(6):102-9. Available from: