Antioxidant and Antinociceptive Activity of Methanol Extract of Leaves of Malus Pumila Mill in Mice
Conventional medications are expensive and arguably associated with various severe adverse effects, hence the need to develop herbal agents that are effective as alternative. Apple (Malus pumila Mill) is the fruit of malus plant. It belongs to the family rosaceae and has been widely cultivated in various parts of the world for centuries. It has a beneficial effect on cardiovascular disease, cancer, pulmonary function and age-related cognitive decline. The leaves of is M. pumila rich in resources, but lack of medicinal value research. Chemical constituents of the same family of M. pumila have many activities. This study evaluated the antioxidant and antinociceptive effect of the methanolic extract of M. pumila leaves in mice. Qualitative phytochemical screening of methanolic extract was carried out to identify the phytoconstituents. The In vitro antioxidant activity of methanolic extracts of M. pumila leaves was assessed against hydrogen peroxide scavenging assay using standard protocols. The antinociceptive activity of methanolic extracts was investigated in thermal-induced (tail immersion) and chemical-induced (formalin) nociception models in mice at two different doses (200 and 400 mg/kg; p.o.). Morphine sulphate (10mg/kg, i.p.) was used as reference analgesic agents. M. pumila extract demonstrated potent and dose-dependent antinociceptive activity in the chemical and heat induced mice models (p < 0.001). The findings of this study indicate that the involvement of both peripheral and central antinociceptive mechanisms. Further, the phytochemical screening results showed that the extract had flavonoids, steroids, saponins, phenolics and terpenoids which have been associated with anti-nociceptive activities. Therefore, the study has established that the methanolic extracts of M. pumila are effective in the management of pain and support the traditional use of this plant in different painful conditions.
Keywords: Malus pumila Mill, Rosaceae, Antioxidant activity, Phytochemical screening, Anti-nociceptive activity
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).