Motility and chemotaxis of Escherichia coli in medium with attractant and repellent

  • Alvin T Reyes
  • Dave Ambita
  • Jamie Louise Batalon
  • Berna Lou Aba
  • Angelbert Cortes
  • Cherray Gabrielle Macabecha
  • Andrew Montecillo

Abstract

This study was conducted in order to satisfy the following objectives: (1) To compare the migration bands of Escherichia coli, Bacillus megaterium and Staphylococcus aureus in Sugar, Indole and Motility (SIM) medium + chemotaxis medium (CM), SIM + attractant (glucose), SIM + repellent (alcohol) and SIM only; (2) To observe and compare the swimming behaviour of E. coli under the microscope in the presence of attractant and repellent; and (3) To compare chemotaxis of E. coli in the presence of attractant and repellent by capillary assay.  Motility bands were observed in tubes with SIM + CM, SIM + attractant, SIM + repellent and SIM only that were inoculated with E. coli and B. megaterium. However, the motility band in SIM + repellent travelled less far as compared to SIM + CM and SIM + attractant. No motility bands were observed in tubes inoculated with S. aureus. Highest number of colonies (CFU/mL) were observed in capillary tubes dipped in attractant (3.7 x 108), followed by CM (5.2 x 107) and least in repellent (7.6 x 106). Analysis of data showed that log CFU/mL in capillary tubes dipped in attractant (8.55±0.14) was significantly higher as compared to capillary tubes dipped in CM (7.64±0.29) and repellent (6.87±0.14). 


Keywords: Escherichia coli, motility, chemotaxis, attractant, repellent

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Author Biographies

Alvin T Reyes

1 University of the Philippines Los Baños, College Laguna Philippines;

2 College of Fisheries-Freshwater Aquaculture Center, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines

Dave Ambita

University of the Philippines Los Baños, College Laguna Philippines;

Jamie Louise Batalon

University of the Philippines Los Baños, College Laguna Philippines;

Berna Lou Aba

University of the Philippines Los Baños, College Laguna Philippines;

Angelbert Cortes

University of the Philippines Los Baños, College Laguna Philippines;

Cherray Gabrielle Macabecha

University of the Philippines Los Baños, College Laguna Philippines;

Andrew Montecillo

University of the Philippines Los Baños, College Laguna Philippines;

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How to Cite
1.
Reyes AT, Ambita D, Batalon JL, Aba BL, Cortes A, Macabecha CG, Montecillo A. Motility and chemotaxis of Escherichia coli in medium with attractant and repellent. JDDT [Internet]. 15Sep.2019 [cited 4Jun.2020];9(5):15-8. Available from: http://jddtonline.info/index.php/jddt/article/view/3532

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