Motility and chemotaxis of Escherichia coli in medium with attractant and repellent
This study was conducted in order to satisfy the following objectives: (1) To compare the migration bands of Escherichia coli, Bacillus megaterium and Staphylococcus aureus in Sugar, Indole and Motility (SIM) medium + chemotaxis medium (CM), SIM + attractant (glucose), SIM + repellent (alcohol) and SIM only; (2) To observe and compare the swimming behaviour of E. coli under the microscope in the presence of attractant and repellent; and (3) To compare chemotaxis of E. coli in the presence of attractant and repellent by capillary assay. Motility bands were observed in tubes with SIM + CM, SIM + attractant, SIM + repellent and SIM only that were inoculated with E. coli and B. megaterium. However, the motility band in SIM + repellent travelled less far as compared to SIM + CM and SIM + attractant. No motility bands were observed in tubes inoculated with S. aureus. Highest number of colonies (CFU/mL) were observed in capillary tubes dipped in attractant (3.7 x 108), followed by CM (5.2 x 107) and least in repellent (7.6 x 106). Analysis of data showed that log CFU/mL in capillary tubes dipped in attractant (8.55±0.14) was significantly higher as compared to capillary tubes dipped in CM (7.64±0.29) and repellent (6.87±0.14).
Keywords: Escherichia coli, motility, chemotaxis, attractant, repellent
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