Motility and chemotaxis of Escherichia coli in medium with attractant and repellent
This study was conducted in order to satisfy the following objectives: (1) To compare the migration bands of Escherichia coli, Bacillus megaterium and Staphylococcus aureus in Sugar, Indole and Motility (SIM) medium + chemotaxis medium (CM), SIM + attractant (glucose), SIM + repellent (alcohol) and SIM only; (2) To observe and compare the swimming behaviour of E. coli under the microscope in the presence of attractant and repellent; and (3) To compare chemotaxis of E. coli in the presence of attractant and repellent by capillary assay. Motility bands were observed in tubes with SIM + CM, SIM + attractant, SIM + repellent and SIM only that were inoculated with E. coli and B. megaterium. However, the motility band in SIM + repellent travelled less far as compared to SIM + CM and SIM + attractant. No motility bands were observed in tubes inoculated with S. aureus. Highest number of colonies (CFU/mL) were observed in capillary tubes dipped in attractant (3.7 x 108), followed by CM (5.2 x 107) and least in repellent (7.6 x 106). Analysis of data showed that log CFU/mL in capillary tubes dipped in attractant (8.55±0.14) was significantly higher as compared to capillary tubes dipped in CM (7.64±0.29) and repellent (6.87±0.14).
Keywords: Escherichia coli, motility, chemotaxis, attractant, repellent
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeÂ The Effect of Open Access).