Evaluation of Phytochemicals in Methanolic Extract of Hypnea musciformis (Wulf.) Lamouroux Collected from Manapad in the South East Coast of Tamil Nadu, India
The present study was carried out to evaluate the phytochemicals in the methanolic extract of Hypnea musciformis (Wulf.) Lamouroux collected from Manapad in the south east coast of Tamil Nadu, India. The phytochemical screening of methanolic extract was studied using the standard procedure by UV-Visible spectroscopic, HPLC and FTIR. The UV-Visible spectrum showed the compounds separated at the nm of 300, 350, 420, 460, 534.5, 538.5, 606.35 646 and 667.5 with the absorption 0.361, 0.249, 0.675, 4.000, 0.967, 0.098, 1.260, 2.460 and 2.571 respectively. The qualitative HPLC fingerprint profile displayed six compounds at different retention time of 1.923min, 2.140min, 2.533min, 3.017min, 3.260min and 3.525min. The result of FTIR analysis was found the presence of functional groups such as amides, phosphorus compound, alcohols, phenols and halogen compounds.
Keywords: Phytochemicals, Hypnea musciformis, UV-Visible, HPLC, FTIR
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