Preliminary Phytochemical and GC-MS Analyses of Methanolic Extract of Blechnum orientale L. Collected From Kothiyar, Kanyakumari District, Tamil Nadu, India
The aim of the study was to screen the phytochemicals of methanolic extract of Blechnum orientale L. collected from Kothiyar, located in Kanyakumari district, Tamil Nadu, India. The preliminary phytochemical analysis was carried out by Harborne method, followed by GC-MS analysis. In the preliminary phytochemical analysis of Blechnum orientale L., the presence of twelve different types of secondary metabolites such as alkaloids, anthocyanin, anthraquinones, cardiac glycosides, coumarin, diterpenes, emodins, flavonoids, saponins, steroids, tannins and triterpenoids were reported in methanolic extract. GC-MS spectrum of methanolic extract of Blechnum orientale L. showed 20 different major peaks which indicated the presence of 20 compounds. The prevailing compounds in methanol extract were toluene (1.927min), thiazole, 4,5-dihydro-2-methyl- (2.249min), ethylbenzene (2.466min), benzene, 1,3-dimethyl- (2.523min), phosphine, acetyldimethyl- (2.684min), guanethidine (4.584min), 2-propanamine, n-methyl-n-nitroso- (5.303min), 4h-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl- (5.435min), diethylene glycol hexyl ether (6.333min), 2,2-dimethylpropanoic acid, nonyl ester (6.655min), 8-thiabicyclo[3.2.1]oct-2-ene (7.156min), 4h-pyrazole, 3-tert-butylsulfanyl-4,4-bistrifluoromethyl- (9.217min), 1,3-benzenediol, 4-propyl- (11.250min), cyclohexanone, 2-methyl-5-(1-methylethenyl)- (13.775min), 2,4-hexadienal, (E,E)- (14.210min), n-hexadecanoic acid (14.966min), phytol (16.451min), 2(1h)-naphthalenone, octahydro-4a-methyl-7-(1-methylethyl)-, (4a.alpha.,7.beta.,8a.beta.)- (16.602min), 7-pentadecyne (16.659min) and 1,4-bis (trimethylsilyl) benzene (20.186min) respectively.
Keywords: Phytochemicals, Pteridophytes, GC-MS, Blechnum orientale, NIST
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