In-vitro Screening for Anthelmintic Potential of Crotalaria retusa on Pheretima posthuma
Objective: Aim of the present study is to evaluate the anthelmintic potential of Crotalaria retusa leaf extract on Pheretima posthuma.
Methods: Extracts of Crotalaria retusa leaves prepared by Soxhlet method. The extracts were screened for phytochemicals such as alkaloid, flavonoid, tannin, steroids, etc. aqueous (AECR) and ethanolic (EECR) extracts were tested for anthelmintic activity using Indian adult earthworm (Pheretima posthuma).
Results: The observation showed that EECR gave shorter paralysis and death time at 100 mg/mL as compared to AECR. Mean±SEM values were calculated for both extract and standard. EECR showed anthelmintic activity in a dose-dependent manner taking the shortest time for paralysis.
Conclusion: The result demonstrated that both the investigational extracts showed significant anthelmintic activity (P<0.05) for the time taken for paralysis and death when compared to standard drug mebendazole.
Keywords: Crotalaria retusa, Anthelmintic, Pheretima posthuma, phytochemical.
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