Isolation and Characterizations of new alkaloid 3-deoxy- 3, 11-epoxy cephalotaxine from Clitoria ternatea
The present study describes the isolation and structural elucidation of new alkaloid 3-deoxy-3, 11-epoxy cephalotaxine (1) from the flowers of C. ternatea. It was isolated by careful column chromatographic separation of the crude extract on silica gel 60. The structure was established based on UV, IR, 1HNMR, 13CNMR and GC-MS spectroscopy methods. The antibacterial activity of various bacterial and fungal strains and anti-inflammatory activities of the isolated compound and its crude methanol extract was studied. The highest zone of inhibition (13.0 and 12.0 mm) was shown by 1 at a dose of 200 µg/kg against E. coli and S. aureus strains and (16.0, 12.0 mm) against anti-fungal strains of C. albicans and A. flavus. The results indicated that at both dose levels (100 mg and 200 mg / kg) of isolated compound 1 had significant anti-inflammatory activity from 2nd hour onwards. The bioactive compound isolated from this plant can be employed for antimicrobial activity, also for the treatment of various bacterial and fungal infections and to show pronounced anti-inflammatory effects after three hours of injection.
Keywords: Clitoria ternatea, Alkaloid, 3-deoxy-3, 11-epoxy cephalotaxine, Anti-microbial activity, Anti-inflammatory activity
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).