Efficacy of partial purified bacteriocin of Pseudomonas aeruginosa on Methicillin-resistant Staphylococcus aureus biofilm

  • Arumugam Thangarasu
  • Dhanam Selvam
  • Sampath Gattu
  • Kayalvizhi Nagarajan Periyar University

Abstract

Biofilms are microbial communities that cause serious chronic infections in the environment by enhancing antimicrobial resistance. Bacteria in the biofilm can be up to a thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. The emergence of antibiotic-resistant microorganism has led to the exploration of different therapeutic agents like ribosomally synthesized microorganism peptides referred to as bacteriocins. In this study, bacteriocin producing bacteria Pseudomonas aeruginosa isolated from a soil sample. It was found to be effective against Methicillin-resistant Staphylococcus aureus (MRSA). Furthermore the bacteriocin was partial purified by ammonium sulfate, the precipitate has highly effective against MRSA (400AU/mL). MRSA cells were treated with precipitated culture supernatant of P. aeruginosa TA6 was analyzed by FT-IR. The treated and untreated MRSA showed band variations at 682.59 and 3442.15cm-1 corresponding to the alkyl and amide group respectively. Bacteriocin showed marked inhibition activity against the biofilm of MRSA. About 0.05% and 0.02% attachment of biofilm was observed in the presence of 1X MIC (10 μg/mL) and 2X MIC (20 g/mL) respectively. Our results recommend that bacteriocins that make stable pores on biofilm cells are extremely potent for the treatment of MRSA biofilm infections.

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Author Biographies

Arumugam Thangarasu

Department of Zoology, School of Life Sciences, Periyar University, Salem-636011, Tamil Nadu, India

Dhanam Selvam

Department of Zoology, School of Life Sciences, Periyar University, Salem-636011, Tamil Nadu, India

Sampath Gattu

Department of Zoology, School of Life Sciences, Periyar University, Salem-636011, Tamil Nadu, India

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How to Cite
Thangarasu, A., Selvam, D., Gattu, S., & Nagarajan, K. (2019). Efficacy of partial purified bacteriocin of Pseudomonas aeruginosa on Methicillin-resistant Staphylococcus aureus biofilm. Journal of Drug Delivery and Therapeutics, 9(4-A), 438-441. https://doi.org/10.22270/jddt.v9i4-A.3464