IN VITRO SULFORHODAMINE B ASSAY EVALUATION OF NOVEL 2-PHENYL BENZOFURANONE DERIVATIVES ON HUMAN SKIN CANCER CELL LINE G361.
The newly synthesized compounds are being tested for in vitro anticancer activity. The method used for In Vitro testing is Sulforhodamine B assay also known SRB assay. Cell lines were prepared and homogenized and disassociated with the help of trypsin. Then trypsin was inactivated with fetal bovine serum. Then cell concentration was determined. Synthesized molecules were prepared into four different dilutions and exposed to cell lines. The procedure was also compared with standard drug doxorubicin. All the cell medium were incubated 37 degrees centigrade in a humidified incubator with 5 percentage CO. The plates were stained and fixed with trichloroacetic acid. Finally, the plates were incubated in orbital shaker incubator and absorbance was measured in a microplate reader at 510nm. All compounds (1-30) showed the similar anticancer activity of compounds (IA, IB, ID, IE, IF, IIB, IIC, IIIA, IVB, IVF, VA, VC, VD, VE.) were more potent when compared to the rest of the compounds synthesized.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).