Simultaneous Estimation and Validation of Multicomponent Formulation in Tablets by Spectrophotometric Method
Two methods for the simultaneous estimation of atorvastatin calcium, glimepiride and metformin hydrochloride in three-component solid dosage forms have been developed. First method involved solving simultaneous equations based on measurement of absorbance at three wavelengths, 247.2 nm, 224.8 nm and 236 nm as the λmax of atorvastatin calcium, glimepiride and metformin hydrochloride respectively while in the second method the instrument is preprogrammed to collect and compile the spectral data from the scan of standards and produces the result by matrix calculations. The linearity for three drugs was in the range of 5-30 μg/ml. The procedures were successfully applied for the simultaneous determination of three drugs in laboratory prepared mixtures and commercial tablet preparation. High recovery and low % COV revealed the reliability of the methods indicating proposed methods are accurate and precise for the simultaneous estimation of three drugs in pure and tablet formulation.
Keywords: Atorvastatin calcium, Glimepiride, Metformin hydrochloride, Simultaneous equation method. Multiwavelength Spectroscopy
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