Simultaneous Estimation and Validation of Multicomponent Formulation in Tablets by Spectrophotometric Method
Two methods for the simultaneous estimation of atorvastatin calcium, glimepiride and metformin hydrochloride in three-component solid dosage forms have been developed. First method involved solving simultaneous equations based on measurement of absorbance at three wavelengths, 247.2 nm, 224.8 nm and 236 nm as the λmax of atorvastatin calcium, glimepiride and metformin hydrochloride respectively while in the second method the instrument is preprogrammed to collect and compile the spectral data from the scan of standards and produces the result by matrix calculations. The linearity for three drugs was in the range of 5-30 μg/ml. The procedures were successfully applied for the simultaneous determination of three drugs in laboratory prepared mixtures and commercial tablet preparation. High recovery and low % COV revealed the reliability of the methods indicating proposed methods are accurate and precise for the simultaneous estimation of three drugs in pure and tablet formulation.
Keywords: Atorvastatin calcium, Glimepiride, Metformin hydrochloride, Simultaneous equation method. Multiwavelength Spectroscopy
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeÂ The Effect of Open Access).