Antioxidant properties and subchronic toxicity of the standardized extract of LAMIC, a phytomedicine prototype based on aqueous extracts from trunk bark of Lannea microcarpa Engl and K. Krause

  • Lazare Belemnaba Research Institute for Health Sciences / National Centre for Scientific and Technological Research (IRSS/CNRST), 03 BP 7047 Ouagadougou 03, Burkina Faso.
  • Madi Soubeiga 2University Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso. 
  • Geoffroy G. Ouédraogo 1Research Institute for Health Sciences / National Centre for Scientific and Technological Research (IRSS/CNRST), 03 BP 7047 Ouagadougou 03, Burkina Faso.
  • Tata Kadiatou Traoré 2University Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso. 
  • Mathieu Nitiéma 1Research Institute for Health Sciences / National Centre for Scientific and Technological Research (IRSS/CNRST), 03 BP 7047 Ouagadougou 03, Burkina Faso. ; 2University Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
  • Sylvain Ilboudo 1Research Institute for Health Sciences / National Centre for Scientific and Technological Research (IRSS/CNRST), 03 BP 7047 Ouagadougou 03, Burkina Faso.
  • B. Mohamed Belemlilga 1Research Institute for Health Sciences / National Centre for Scientific and Technological Research (IRSS/CNRST), 03 BP 7047 Ouagadougou 03, Burkina Faso. ; 2University Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
  • Souleymane Compaoré 1Research Institute for Health Sciences / National Centre for Scientific and Technological Research (IRSS/CNRST), 03 BP 7047 Ouagadougou 03, Burkina Faso. ; 2University Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
  • Raïnatou Sondé/Boly 1Research Institute for Health Sciences / National Centre for Scientific and Technological Research (IRSS/CNRST), 03 BP 7047 Ouagadougou 03, Burkina Faso.
  • Jean Claude Romaric Pingwendé Ouédraogo Research Institute for Health Sciences / National Centre for Scientific and Technological Research (IRSS/CNRST), 03 BP 7047 Ouagadougou 03, Burkina Faso. ; 2University Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
  • Salfo Ouédraogo 1Research Institute for Health Sciences / National Centre for Scientific and Technological Research (IRSS/CNRST), 03 BP 7047 Ouagadougou 03, Burkina Faso. ; 2University Pr Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
  • Alphonsine Ramdé/Tiendrebéogo 1Research Institute for Health Sciences / National Centre for Scientific and Technological Research (IRSS/CNRST), 03 BP 7047 Ouagadougou 03, Burkina Faso.
  • Noufou Ouédraogo 1Research Institute for Health Sciences / National Centre for Scientific and Technological Research (IRSS/CNRST), 03 BP 7047 Ouagadougou 03, Burkina Faso.
  • Moussa Ouédraogo 1Research Institute for Health Sciences / National Centre for Scientific and Technological Research (IRSS/CNRST), 03 BP 7047 Ouagadougou 03, Burkina Faso.
  • Sylvin Ouédraogo 1Research Institute for Health Sciences / National Centre for Scientific and Technological Research (IRSS/CNRST), 03 BP 7047 Ouagadougou 03, Burkina Faso.

Abstract

Aims: This study investigated the antioxidant activity and the 90 days subchronic toxicity of the standardized LAMIC phytomedicine prototype based on aqueous extracts from Lannea microcarpa trunk bark.


Methods: Three spectrophotometric methods were used to evaluated the antioxidant activity of LAMIC which were 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical, 2,2’-azinobis(3-ethylbenzolin-6-sulphonate) (ABTS) radical scavenging assays and ferric reducing antioxidant power (FRAP) assays. For the standardized LAMIC subchronic toxicity study, male and female Wistar rats were used by daily oral administration at doses of 500, 1000 and 1500 mg/kg bw consecutively for 90 days.


Results: The LAMIC extract exhibit better inhibitory activity against DPPH radical than ABTS radical with respective IC50 values of 45.38±3.21 µg/mL and 66.45±18.76 µg/mL, while FRAP assay exhibit antioxidant activity of 211.34±15.92 mmol EAA/g.


Subchronic oral administration of LAMIC was well-tolerated at all tested doses. No behavioral and physiological changes and mortality were observed. The LAMIC extract did not present any impact on general hematological parameters and biochemical parameters. Moreover, no significant changes were raised in organ and body weight of treated groups compared to the Control group.


Conclusion: These results support that LAMIC prototype was a valuable source of natural antioxidants and no toxicity was associated to its long terms oral consumption in rats indicating a potential application as a cardiovascular protective formulation.


Keywords: LAMIC–Lannea microcarpa–Standardization–Antioxidant–Subchronic toxicity.


 

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How to Cite
Belemnaba, L., Soubeiga, M., Ouédraogo, G. G., Traoré, T. K., Nitiéma, M., Ilboudo, S., Belemlilga, B. M., Compaoré, S., Sondé/Boly, R., Ouédraogo, J. C. R. P., Ouédraogo, S., Ramdé/Tiendrebéogo, A., Ouédraogo, N., Ouédraogo, M., & Ouédraogo, S. (2019). Antioxidant properties and subchronic toxicity of the standardized extract of LAMIC, a phytomedicine prototype based on aqueous extracts from trunk bark of Lannea microcarpa Engl and K. Krause. Journal of Drug Delivery and Therapeutics, 9(5), 1-8. https://doi.org/10.22270/jddt.v9i5.3285