Formulation of Coffee Bean Extract (Chlorogenic Acid) Solid Lipid Nanoparticles for Lymphatic Uptake on Oral Administration
Coffee bean extract (Chlorogenic Acid), Disease Modifying Anti-Rheumatoid Drug (DMARD) agent in the treatment of Rheumatoid Arthritis is believed to inhibit the production of TNF-α by blocking the cell associated conversion of TNF Precursor to mature proteins this, halting the proliferation of synovitis.CGA inhibit the proliferation of the fibroblast‑like synoviocyte cell line (RSC‑364), stimulated by interleukin (IL)‑6, through inducing apoptosis. CGA inhibit the inflammatory proliferation of RSC‑364 cells mediated by IL‑6 through inducing apoptosis. CGA was also able to suppress the expression levels of key molecules in the JAK/STAT and NF‑κB signaling pathways, and inhibit the activation of these signaling pathways in the inflammatory response through IL‑6‑mediated signaling, thereby resulting in the inhibition of the inflammatory proliferation of synoviocytes. The aim of the present study was to formulate and evaluate coffee bean extract (chlorogenic acid) solid lipid nanoparticles using a positive charge on it by means of enabling lymphatic uptake. Coffee bean extract (chlorogenic acid) solid lipid nanoparticles were prepared by melt emulsification-high pressure homogenization method and the particle size, PDI and % entrapment efficiency was found to be 210nm, 0.455 and 91.18% .ex vivo endocytic uptake studies revealed engrossment of endocytic pathways in the uptake of solid lipid nanoparticles from intestine.
Keywords: Coffee bean extract (Chlorogenic Acid), Glyceryl monostearate, Solid Lipid Nanoparticles, Lymphatic Uptake
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