PROTEIN RICH BIOMASS PRODUCTION BY NOVEL YEAST Cyberlindnera jadinii MMS7 USING Chara sp. HYDROLYSATE AND EVALUATION OF ITS ANTI-BACTERIAL ACTIVITY
A total of seven cultivable purified yeast phenotypes were isolated from soil collected from sugarecane bagasse dumping sites. By the genotypic characterization, new isolate MMS7 was confirmed as most promising yeast strain and it was identified as Cyberlindnera jadinii MMS7. We used this strain to investigate the feasibility of biomass production using hydrolysate of green Chara sp as sole medium. The ultrasonic pretreatment of the Chara sp biomass for hydrolyaste preparation was optimized as 0.35 WmL-1 (ultrasound power density) and 15 min (treatment time) using response surface methodology (RSM). The biomass of 15.05±0.12 g dwL-1 and protein concentration of 59.21±0.12% were obtained in C. jadinii MMS7, when cultivated in hydrolyaste. This study demonstrates the novel yeast C .jadinii MMS7 could be considered a promising single cell protein producing strain using Chara sp hydrolysate and it has high antibacterial activity against Bacillus cereus. Further, the exploitation of Chara sp. hydrolysate potential may used to aggregate value for algae cultivation.
Keywords: Biomass, Cyberlindnera jadinii, Hydrolysate, Pretreatment, Chara sp
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