Evaluation of in vitro anti snake venom activity of Justicia adhatoda leaves extract against Russell’s viper snake venom

  • Ramaswamy Malathi Research Department of Biotechnology, Bharathidasan University Constituent College, Kurumbalur, Perambalur- 621 107, Tamilnadu, India.
  • Dhandapani Kaviyarasan Bharathidasan university
  • Solaimuthu Chandrasekar Research Department of Biotechnology, Bharathidasan University Constituent College, Kurumbalur, Perambalur- 621 107, Tamilnadu, India.

Abstract

Venomous snake bites possess significant amount of mortality as well as morbidity in tropical and subtropical regions of the world including India. The snake Russell’s viper (Daboia russelii) is responsible for thousands of deaths in India.  Although antivenom are the only treatment available and the effects associated with several side effects. As an alternative, plants have been extensively studied in order to obtain an alternative treatment. The uses of plants against the effects of snake bites have been long recognized more scientific attention has been given since last 20 years. Extracts from plants have been used among traditional healers, especially in tropical areas there are plentiful sources, as therapy for snakebite for a long time. The present study aimed to provide a scientific explanation for the use of Justicia adhatoda plant against snakebite. The acetone extract of Justicia adhatoda leaves were tested for in vitro inhibitory activity on toxic venom enzymes like 5' nucleotidase, L-amino acid oxidase, phosphomonoesterase, phosphodiesterase, acetylcholine esterase and hyaluronidase from the Russell’s viper snake venom. The result showed the potential of plant leaves extract against the enzymes present in the snake venom. Justicia adhatoda inhibits most of the snake venom enzymes like L-amino acid oxidase, phosphomonoesterase, phosphodiestarase and acetylcholine esterase.


Keywords: Daboia russelii, Enzyme inhibition, Acetone Extract of Justicia adhatoda (AEJA), etc.

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Author Biographies

Ramaswamy Malathi, Research Department of Biotechnology, Bharathidasan University Constituent College, Kurumbalur, Perambalur- 621 107, Tamilnadu, India.

Research Department of Biotechnology, Bharathidasan University Constituent College, Kurumbalur, Perambalur- 621 107, Tamilnadu, India.

Dhandapani Kaviyarasan, Bharathidasan university

Research Department of Biotechnology, Bharathidasan University Constituent College, Kurumbalur, Perambalur- 621 107, Tamilnadu, India

Solaimuthu Chandrasekar, Research Department of Biotechnology, Bharathidasan University Constituent College, Kurumbalur, Perambalur- 621 107, Tamilnadu, India.

Research Department of Biotechnology, Bharathidasan University Constituent College, Kurumbalur, Perambalur- 621 107, Tamilnadu, India.

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How to Cite
Malathi, R., Kaviyarasan, D., & Chandrasekar, S. (2019). Evaluation of in vitro anti snake venom activity of Justicia adhatoda leaves extract against Russell’s viper snake venom. Journal of Drug Delivery and Therapeutics, 9(4), 116-122. https://doi.org/10.22270/jddt.v9i4.3116