Isolation, Screening and Identification of Potential Thermo Stable Bacterial Enzyme Producers in Sangameshwar, Tural Hot Spring
The present study aimed to isolate, characterize and screen the potential enzyme producing bacteria from the sediment and water samples collected from the Sangameshwar-Tural hot spring having 63OC temperature. This study helps to explore the unexplored bacterial diversity within the Sangameshwar-Tural hotsprings. Twelve isolates were obtained from both sediment and water samples were subjected to molecular characterization for the identification of hot spring bacterial isolates. All the isolates obtained from this study were further subjected to enzyme screening; the enzymes were protease, amylase, lipase, keratinase and urease. The enzyme urease alone was produced from both sediment and water sample isolates whereas the other enzymes were not produced. In Sangameshwar-Tural sediments out of nine isolates, five isolates were positive and in Sangameshwar-Tural water, one isolate were positive out of three isolates.
Keywords: DNA isolation, Hot Springs, 16S rRNA gene sequencing, Enzyme screening
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