Induction of Apoptosis and Antiproliferative Activity of Alangium salviifolium against Non-melanoma and Melanoma Cancer Cells

  • Preeti Dhruve 1Radiation and Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi - 110067, India
  • Raosaheb K. Kale Radiation and Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi - 110067, India
  • Rana P. Singh Radiation and Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi - 110067, India

Abstract

Ayurveda is a traditional medicine of system in India which includes plants and plant-based products for treatment and cure of diseases. Alangium salviifolium is an ethnomedicinal plant and has been reported to be useful against skin cancer. In the present study, we used thee concentrations of methanolic bark extract of A. salviifolium (MBEAS) to evaluate its anticancer activity against epidermoid carcinoma A431cells and melanoma B16F10 cells. Assays performed were trypan blue dye exculsion to assess the cytotoxicity, colony formation for cell growth and AO/EtBr staining to check the apoptotic/necrotic effect of A. salviifolium on skin cancer cells. Exposure of cells to A. salviifolium for 24 h and 48 h strongly reduced the cell number of  A431 and B16F10 cells, inhibited the colony formation ability and induced apoptosis in a concentration-dependent manner. The results from the study suggest the antiproliferative activity of A. salviifolium against both melanoma and non-melanoma skin cancer cells. Overall, it provides the needed scientific validity to its traditional use against skin cancer.


Keywords: Anticancer, ethnomedicine, antiproliferative, non-melanoma, melanoma.

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Author Biographies

Preeti Dhruve, 1Radiation and Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi - 110067, India

1Radiation and Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi - 110067, India

Raosaheb K. Kale, Radiation and Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi - 110067, India

Radiation and Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi - 110067, India

Rana P. Singh, Radiation and Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi - 110067, India

Radiation and Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi - 110067, India

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How to Cite
Dhruve, P., Kale, R. K., & Singh, R. P. (2019). Induction of Apoptosis and Antiproliferative Activity of Alangium salviifolium against Non-melanoma and Melanoma Cancer Cells. Journal of Drug Delivery and Therapeutics, 9(4), 426-432. https://doi.org/10.22270/jddt.v9i4.3078