Bioanalytical Method Development and Validation for Estimation of Daunorubicin and Cytarabine in Blood Plasma by Using RP-HPLC

  • Ashish Agrawal
  • Manoj Sharma

Abstract

A simple, sensitive and selective RP-HPLC method was developed for estimation of Daunorubicin and Cytarabine in combined formulation by isocratically using 20 mM KH 2 PO 4 : acetonitrile (pH 2.5) in the ratio of 20:80 v/v as mobile phase, Prontosil C-18 column (4.6 x 250mm, 5μparticle size) column as stationary phase and chromatogram was recorded at 254nm. The validation studies are carried out and it’s fulfilling ICH requirements. The method is found to be specific, linear, precise (including both intra- and inter- day precision), accurate and robust. This proposed method may represent a valuable aid in the laboratory monitoring of the toxicity of anticancer chemotherapy.


Keywords: RP-HPLC, Leukemia, Daunorubicin and Cytarabine, Linearity and Calibration curve

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Author Biographies

Ashish Agrawal

Research Scholar, Bhagwant University, Ajmer (Rajesthan), India

Manoj Sharma

School of Studies in Pharmaceutical Sciences, Jiwaji University, Gwalior, India

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How to Cite
Agrawal, A., & Sharma, M. (2019). Bioanalytical Method Development and Validation for Estimation of Daunorubicin and Cytarabine in Blood Plasma by Using RP-HPLC. Journal of Drug Delivery and Therapeutics, 9(4), 366-373. https://doi.org/10.22270/jddt.v9i4.3061