Bioanalytical Method Development and Validation for Estimation of Daunorubicin and Cytarabine in Blood Plasma by Using RP-HPLC
A simple, sensitive and selective RP-HPLC method was developed for estimation of Daunorubicin and Cytarabine in combined formulation by isocratically using 20 mM KH 2 PO 4 : acetonitrile (pH 2.5) in the ratio of 20:80 v/v as mobile phase, Prontosil C-18 column (4.6 x 250mm, 5μparticle size) column as stationary phase and chromatogram was recorded at 254nm. The validation studies are carried out and it’s fulfilling ICH requirements. The method is found to be specific, linear, precise (including both intra- and inter- day precision), accurate and robust. This proposed method may represent a valuable aid in the laboratory monitoring of the toxicity of anticancer chemotherapy.
Keywords: RP-HPLC, Leukemia, Daunorubicin and Cytarabine, Linearity and Calibration curve
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeÂ The Effect of Open Access).