Development and Validation of RP-HPLC Method for Estimation of Etizolam in Bulk
The present research work describes a simple, accurate, precise, effective, stability indicating, RP-HPLC method for estimation of Etizolam in there tablet dosage form. A Reverse phase high performance chromatographic method was developed for estimation of Etizolam dosage. The separation was achieved by Cosmosil C18 (250mm×4.6ID, Particle size: 5 micron) and methanol: water in the proportion of 30:70v/v as mobile phase, at a flow rate 0.8ml/min. Detection was carried out at 242nm. For RP-HPLC method results of the validation indicate that the method was linear in the range of 100-600 µg/ml for Etizolam. The % recoveries for Etizolam obtained in the accuracy study were 99.77-99.51% respectively. The LOD for Etizolam were found to be 0.7877µg/ml. LOQ for Etizolam found to be 2.382µ/ml. Developed methods were found to be accurate, precise, rapid and stability indicating for estimation of Etizolam.
Keywords: Etizolam, RP-HPLC, tablet dosage form
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