Optimization of Ultrasound-Assisted extraction for Tephrosia purpurea by Response Surface Methodology and evaluation of its antioxidant activity
Objective: The aim of the present research work is to optimize the Ultrasound Assisted Extraction (UAE) of whole plant of Tephrosia purpurea by Response Surface Methodology (RSM) and to evaluate its antioxidant activity by DPPH radical scavenging assay and Ferric (Fe3+) Reducing Power Assay.
Methods: T. Purpurea was subjected to UAE by Box-Behnken experimental design using independent factors/variables. The design matrix developed by software consists of 15 runs. All batches were subjected to DPPH radical power assay to determined antioxidant activity.
Results: The optimal conditions for batch which showed highest extraction yield i.e. 9.82 % were 30 min irradiation time, 15 min soaking time, and 6.665% solid: solvent ratio. Statistical analysis of experiments indicated that the irradiation time and solid: solvent ratio has significantly affected the extraction (p < 0.01). The Box-Behnken experimental design shows that the polynomial regression models are in good agreement with the experimental results and with the coefficients of multiple determination of 0.9499 for extraction yield. The batch showing highest extraction yield were also showed highest DPPH scavenging activity i. e.87.96% and batch having optimal conditions viz 10 min irradiation time, 15 min soaking time, and 6.665% solid: solvent ratio showed 98.08 % ferric reducing power assay.
Conclusion: This research work showed the UAE can be a method of extraction of T. purpurea. The antioxidant activity done by DPPH radical scavenging assay and Ferric (Fe3+) Reducing Power Assay may be useful for further research work of T. purpurea.
Keywords: Tephrosia purpurea, Ultrasound Assisted Extraction, Response surface methodology, Antioxidant activity
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