In-Vitro Antioxidant Activity and Free Radical Scavenging Potential of Phlorizin Derived Sodium Glucose Cotransporter 2 Inhibitor
Objectives- In vitro antioxidant activity assay is the preliminary step to determine a drug’s efficacy in combating oxidative stress when used in a clinical condition. The current study was aimed to determine the in vitro antioxidant activity of antidiabetic drug-SGLT-2 inhibitor (canagliflozin, dapagliflozin and empagliflozin) in combating the oxidative stress in diabetes mellitus. Methods- A total of five methods were adopted for determining the antioxidant potential of the drugs. The methods were DPPH radical scavenging assay, nitric oxide radical scavenging assay, phosphomolybdenum assay, assessment of inhibition of lipid peroxidation and FRAP. Results- The results indicated canagliflozin as best DPPH radical scavenger and empagliflozin as the best scavenger of nitric oxide radicals. Also, empagliflozin showed best reducing power and canagliflozin showed promising results in inhibiting lipid peroxidation. Conclusion- These findings suggest that use of canagliflozin and empagliflozin in diabetic patient will control the hyperglycemic conditions well as other complications that are caused by hyperglycemia induced oxidative stress.
Keywords- DPPH radicals, Lipid peroxidation, Oxidative stress, Phosphomolybdenum assay, SGLT-2 inhibitor
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