Protective Activity of Tinospora cordifolia and Camellia sinensis extract on Alcohol-Induced Toxicity in Mice
Heavy alcohol intake depletes the plasma vitamins due to hepatotoxicity and decreased intestinal absorption. Medicinal plants may provide an effective remedy for the enormous health burden posed by alcohol abuse. This study was designed to observe the effects of alcohol on various hematological parameters in adult mice. A total of 30 mice were divided into six groups; control (n=6) and test groups II, III, IV, V, VI (n=6 comprise in each group). After treatments blood was collected on 0th, 30th and 60th days. Hematological parameters were assessed by using bioanalyzer. Statistical analysis was performed for comparison between the groups by using Dunnett’s test. The white blood cells (WBC) was found significantly higher in a alcohol treated group compared to control group, while the significant decrease was observed in hemoglobin, hemocrait and platelets in the alcohol treated group. In contrast the differential count viz. neutrophil, eosinophil, monocyte, lymphocyte, basophil were found significant (p≤0.05) compared to control group and test groups II, III, IV, V and V respectively. However, administration of extract for 60 days was capable to slightly lower levels of all these parameter in alcohol groups. It is concluded that alcohol administration in mice resulted in toxic effects on various hematological parameters. T. cordifolia and leaf part of C. Sinensis helps more to wakeup immune system and boost the body of person who regularly consume alcohol.
Keywords: Alcohol, Hematological parameters, Dunnett’s test
2. WHO. Global status report on alcohol and health. 2004; Geneva.
3. Sarkar MK, Sarkar K, Bhattacharjee R, Chatterjee M, Sil PC. Curative role of the aqueous extract of the herb, phyllanthus niruri, against nimesulide induced oxidative stress in murine liver. Biomedical Res 2005; 16: 171-176.
4. Mandayam S, Jamal MM, Morgan TR. Epidemiology of alcoholic liver disease. Semin Liver Dis 2004; 24: 217-232.
5. AlbanoE, French S, Ingelman-Sundberg M. Cytochrome p450 2E1, hydroxyl ethyl radicals, and immune reaction associated alcoholic liver injury. Alcoh Clini Exper Res 1994; 18:1057-1068.
6. Zaidi SMKR, Al-Qirim TM, Banu N. Effects of antioxidant vitamins on glutathione depletion and lipid peroxidation induced by restraint stress in the rat liver. Drugs in R & D 2005; 6(3):157-165.
7. King J. The transferase alanine and aspartate transaminase in Practical Clinical Enzymology Nostrand, London, UK, 1965; pp 363-395.
8. Bun SS, Bun H, Guedon D, Rosier C, Ollivier E, Effect of green tea extracts on liver functions in Wistar rats. Food Chem Toxicol 2006; 44(7): 1108-1113.
9. Cai Y, Ma L, Hou L, Zhou B, Yang L, Liu Z. Antioxidant effects of green tea polyphenols on free radical initiated peroxidation of rat liver microsomes. Chem Physics Lipids 2002; 120 (1-2)109-117.
10. Jin X, Zheng R-H, Li Y-M. Green tea consumption and liver disease: a systematic review. Liver Int 2008; 28(7): 990-996.
11. Anonymous. Wealth of India: A dictionary of Indian Raw Materials and Industrial Products. 1st ed. Vol X. New Delhi: CSIR; 2003. pp. 251-2.
12. Aima RK. Pictorial Guide to Plants. 1st ed. Dehradun: Natraj Publishers. 2003. pp. 454-5.
13. Vaidya DB. Materia Medica of Tibetan Medicine. Delhi: Sri Satguru Publications; 1994. pp. 163.
14. Bhandari C. Vanaushadhi Chandrodaya. 1st ed., Vol. 3. Varanasi: Chaukhamba Sanskrit Sansthan; 2006. pp. 86.
15. Singh SS, Pandey SC, Srivastava S, Gupta VS, Patro B, Ghosh AC. Chemistry and medicinal properties of Tinospora cordifolia (Guduchi). Indian J Pharmacol 2003; 35: 83-91.
16. Panchabhai TS, Kulkarni UP, Rege NN. Validation of therapeutic claims of Tinospora cordifolia: a review. Phytother Res 2008; 22: 425-441.
17. Sharma U, Bala M, Kumar N, Singh B, Munshi RK, Bhalerao S. Immunomodulatory active compounds from Tinospora cordifolia. J Ethnopharmacol 2012; 141; 918-926.
18. Desai VR, Ramakrishnan R, Chintalwar GT, Sainis KR. G1-4A, an immunomodulatory polysaccharide from Tinospora cordifolia, modulates macrophage responses and protects mice against lipopolysaccharide induced endotoxic shock. Int Immunopharmacol 2007; 7: 1375-1386.
19. Gupta PK, Chakraborty P, Kumar S et al. G1-4A, a polysaccharide from Tinospora cordifolia inhibits the survival of Mycobacterium tuberculosis by modulating host immune responses in TLR4 dependent manner. PLoS One 2016; 11: e0154725.
20. Chaudhry R, Jahan S, Goyal PK. Chemopreventive potential of an Indian medicinal plant (Tinospora cordifolia) on skin carcinogenesis in mice. J Environmen Pathol Toxicol Oncol 2008; 27: 233-243.
21. Khandelwal KR. Practical Pharmacognosy Techniques and Experiments (Nirali Prakashan Pune, 2000) pp. 149-156.
22. Dacie JV, Lewis SM. Practical Haematology 5th ed. Churchill Livingstone, London, 1975; pp. 502- 503.
23. Alexander RR, Grifiths JM. Haematocrit determination by the cyanomethaemoglobin method in: Biochemical Methods, 2nd ed., John Wiley and Sons, Inc. Publications, New York, 1987; pp.186- 187.
24. Osim EE, Akpogomeh BA, Ibu JO, Eno AE Experimental Physiology Manual, Department of Physiology, University of Calabar, Calabar 3rd ed. 2004; pp. 60- 81.
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