In-Vitro Evaluation of Drug Release and Antioxidant Activity of Aloe Loaded Chitosan Nanoparticles
Antioxidants play significant responsibility during the treatment of cancer and it’s adverse effects, because in the treatment of cancer by using chemotherapy, free radicals are generated and these free radicals accumulated in the body and cause damage, side effect (such as neurodegenerated disease, atherosclerosis, depression, leukemia, skin disease, diabetes etc.) and even death due to the biological consequence. So, antioxidant inhibits the generation of new and destroys previously present free radicals. A recent study was aimed for novel approach of herbal nanoparticles to get improved efficacy of the drug with fewer side effects. In-vitro Evaluation of Drug Release and Antioxidant Activity of Aloe Loaded Chitosan Nanoparticles were includes in this research. Aloe has good antioxidant property, but in the form of nanoparticles formed with the help of chitosan polymer, it’s enhancing their stability, in-vitro release property. Chitosan is one of the best polymer for nanomedicine formulation because of their low toxicity and high compatibility. Six formulations of aloe loaded chitosan nanopaticles were prepared and evaluated as antioxidant study, in-vitro release and stability studies. Results have shown that the F6 was most stable, good release and high antioxidant property. So, it found that F6 was good formulation among all batches and these nanoparticles can be used as antioxidant agent during the treatment of cancer along with chemotherapeutic drugs for improve their effectiveness and reduce adverse effects.
Keywords: Cancer, Chemotherapy, Herbal, Chitosan nanoparticles, Antioxidant.
Patel Jitendra S, Bhatt Medha M, Patel Foram A, Dhoru Minakshi M, Patel Madhabhai M, Nanotechnology: A New Approach In Herbal Medicine, American Journal of Pharmtech Research, 2013; 3(4):275-288.
2. Mathur Manish, Vyas Govind, Role of nanoparticles for production of smart herbal drug- An overview, Indian Journal of Natural Products and Resources, 2013: 4(4):329-338.
3. Sharma Parth, Verma Surajpal, Misri Plakshi, Global Need for Novel Herbal Drug Formulations, International Journal of Pharmacognosy and Phytochemical Research, 2016; 8(9):1535-1544.
4. Ravi Krittika, Ganapathy Dhanraj, Sherlyn Sheeba P, Antioxidants and cancer prevention - A review, Journal of Pharmacy Research, 2018; 12(1):35-38.
5. Nuria Mut-Salud, Pablo Juan Álvarez, Jose Manuel Garrido, Esther Carrasco, Antonia Aránega, and Fernando Rodríguez-Serrano, Antioxidant Intake and Antitumor Therapy: Toward Nutritional Recommendations for Optimal Results, Oxidative Medicine and Cellular Longevity, 2016; 1(1):1-19.
6. Ray Sarbani Dey, Potential aspects of chitosan as pharmaceutical excipients, Acta Poloniae Pharmaceutica - Drug Research, 2011; 68(1):619-622.
7. Yashwanth Radhakrishnan, Ganesh Gopal, Chittur C Lakshmanan and Krishna S Nandakumar, Chitosan Nanoparticles for Generating Novel Systems for Better Applications: A Review, Journal of Molecular and Genetic Medicine, 2015; 48:1-10.
8. Harlev Eli, Nevo Eviatar, Lansky Ephraim P, Ofir Rivka, Bishayee Anupam, Anticancer Potential of Aloes: Antioxidant, Antiproliferative, and Immunostimulatory Attributes, Planta Med, 2012; 78:843–852.
9. Hussain Arif, Sharma Chhavi, Khan Saniyah, Shah Kruti, Haque Shafiul, Aloe vera Inhibits Proliferation of Human Breast and Cervical Cancer Cells and Acts Synergistically with Cisplatin, Asian Pacific Journal of Cancer Prevention, 2015;16 (7):2939-2946.
10. Sampath Kumar KP, Bhowmik Debjit, Chiranjib, Biswajit, Aloe vera : A Potential Herb and its Medicinal Importance, Journal of Chemical and Pharmaceutical Research , 2010; 2(1): 21-29.
11. Rajeswari R, Umadevi M, Sharmila Rahale C, Pushpa R, Selvavenkadesh S, Sampath Kumar K P, Bhowmik Debjit, Aloe vera: The Miracle Plant Its Medicinal and Traditional Uses in India, Journal of Pharmacognosy and Phytochemistry, 2012; 1(4):118-124.
12. Sanders Brian, Ray Anna M, Goldberg Sharon, Clark Tyler, Reginald McDaniel H, Atlas Steven E, Farooqi Ashar, Konefal Janet, Lages Lucas C, Lopez Johanna, Rasul Ammar, Tiozzo Eduard, Woolger Judi M, Lewis John E, Anti-cancer effects of aloe-emodin: A systematic review, Journal of Clinical and Translational Research, 2017; 3(4):1-14.
13. Lucini Luigi, Pellizzoni Marco, Molinari Gian Pietro, Franchi Fabrizio, Aloe anthraquninones against cancer, Medical and Aromatic Plant Science and Biotechnology, 2012; 6(2):20-24.
14. Abd El-Aziz Abeer Ramadan Mohamed, Al-Othman Monira Rashed, Mahmoud Mohamed Abobakr, Shehata Shereen Mohamed and Abdelazim Nahla Shazli, Chitosan nanoparticles as a carrier for Mentha longifolia extract: synthesis, characterization and antifungal activity, 2018; 114(10):2116-2122.
15. Khan A, Shaheen A, Mahmood T, Rehman W and Yaqoob, Novel Synthesis and Characterization of Silver Nanoparticles from Leaf Aqueous Extract of Aloe Vera and their Anti-Microbial Activity, Journal of Nanoscience and Nanotechnology Applications, 2017; 1(1):1-9.
16. Praseena K and Junise V “Formulation and evaluation of chitosan nanoparticle loaded with phyllanthin” 2018; 5(04):2644-2649.
17. Salim Khan, Fahad Al-Qurainy, Mauji Ram, Sayeed Ahmad and Malik Zainul Abdin, Phyllanthin biosynthesis in Phyllanthus amarus: Schum and Thonn growing at different altitudes, Journal of Medicinal Plants Research, 2010; 4(1):41-48.
18. Hoa LTM, Chi NT, Triet NM, Nhan LNT and DM, Preparation of drug nanoparticles by emulsion evaporation method, J Phy Conf Ser.2009; 187;1-4.
19. Chitravadivu C, Manian S and Kalaichelvi K, Qualitative Analysis of Selected Medicinal Plants, Tamilnadu, India, Middle-East Journal of Scientific Research, 2009; 4 (3): 144-146.
20. Wu Y, Luo Y and Wang Q, Antioxidant and antimicrobial properties of essential oils encapsulated in zein nanoparticles prepared by liquid dispersion method, LWT. Food Sci. Technol., 2012; 48:283–290.
21. Da Silva SB, Amorim M, Pedro F, Madureira R, Ferreira D, Pintado M, Sarmento B, Natural extracts into chitosan nanocarriers for rosmarinic acid drug delivery, Pharm. Biol., 2015;53:642–652.
22. Kulani MJ, Chitosan nanoparticles functionalized with methanol medicinal plant extracts for the inhibition of the toxic effects of aflatoxin B1 and ochratoxin A, Master Thesis, University of Johannesburg, 2013, p. 97
23. Shah M, Solid Lipid Nanoparticles of a Water Soluble Drug, Ciprofloxacin Hydrochloride, Indian J Pharm Sci., 2012; 74(5):434–442.
24. Pisoschi Aurelia Magdalena, Negulescu Gheorghe Petre, Methods for Total Antioxidant Activity Determination: A Review, Biochem & Anal Biochem, 2011;1(1):1-10.
25. Kumawat Bhupendra K, Gupta Mahesh, Singh Tarachand, Singh Yogendra, Free radical scavenging effect of various extracts of leaves of Balanites aegyptiaca(L.) Delile by DPPH method, Asian Journal of Plant Science and Research, 2012; 2(3):323-329.
26. Phromsopha T, Chitosan microparticles prepared by water in oil emulsion solvent diffusion method for drug delivery, Biotechnology, 2010; 9(1): 61-66.
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