Development and Characterization of Morin Loaded Phytosomes for its Anti-Oxidant Activity

  • Kanika Manral Invertis Institute of Pharmacy, Invertis University Bareilly Uttar Pradesh
  • Arun Kumar Singh Amrapali Institute of Pharmacy and Sciences, Shiksha Nagar, Haldwani Nainital Uttarakhand, India
  • Vibha Sah Formulation and Development Department, Bal Pharma Limited ,Rudrapur U.S.Nagar, Uttarakhand

Abstract

The herbal plants contain huge number of active constituents, which shows excellent biological activity in-vitro but less in-vivo because of their poor lipid solubility, improper molecules size, degradation in gastric fluid and destruction of active components in gut by bacteria. So that poor absorption and bioavailability may occur. Phytosomal technique can be applied to herbal actives for the enhancement of bioavailability of herbal extracts and phytoconstituents. Phytosomes are prepared by patented process where standardized plant extract are bound to phospholipid. The aim of the present study was to developed and characterized the phytosomes of Morin to overcome the limitation of absorption and bioavailability. Phytosomes of Morin was formulated by thin film hydration method by using in different ratio of lipid with phytoconstituents. The prepared phytosomes were characterized for their drug content, drug entrapment, particle size, in-vitro release study and evaluated its in vitro antioxidant activity. From the present study, the result showed that spherical shape phytosomes having particle size 661.2nm. The poly-dispersity index and zeta potential was found to be 0.383 and -13.76 mv respectively. Result obtained from in-vitro release studies shows a sustained release of morin from formulation over a period of 12 hrs. From the present study it was concluded that morin loaded phytosmes shows better absorption and more effective antioxidants than the parent compound.


Keyword: Phytosomes, phospholipids, Morin, in-vitro Antioxidant activity

Downloads

Download data is not yet available.

Author Biographies

Kanika Manral, Invertis Institute of Pharmacy, Invertis University Bareilly Uttar Pradesh

Invertis Institute of Pharmacy, Invertis University Bareilly Uttar Pradesh

Arun Kumar Singh, Amrapali Institute of Pharmacy and Sciences, Shiksha Nagar, Haldwani Nainital Uttarakhand, India

Amrapali Institute of Pharmacy and Sciences, Shiksha Nagar, Haldwani Nainital  Uttarakhand, India

Vibha Sah, Formulation and Development Department, Bal Pharma Limited ,Rudrapur U.S.Nagar, Uttarakhand

Formulation and Development Department, Bal Pharma Limited ,Rudrapur U.S.Nagar, Uttarakhand

References

1. Singh R.P, Parpani S,Narke R,Chavan R. Phytosomes:Recent advance research for novel drug delivery system,Asian journal of pharmaceutical research and development, 2014; 2(3): 15-29.
2. Mishra N, Yadav N P, Meher JG, Sinha P. Phyto-vesicles: conduit between conventional and novel drug delivery system, Asian Pacific Journal of Tropical Biomedicine, 2012; 3(1):1728-1734.
3. Khan J , Alexander A , Ajazuddin , Saraf S , Saraf S. Luteolin–phospholipid complex preparation, characterization and biological evaluation Journal of Pharmacy And Pharmacology, 2014; 4(3):1-12.
4. Khan J, Alexander A, Ajazuddin, Saraf S, Saraf S. Recent advances and future prospects of phyto-phospholipid complexation technique for improving pharmacokinetic profile of plant actives, Journal of Controlled Release, 2013; 168: 50–60.
5. Al-Numair KS, Chandramohan G, C. Veeramani, and M.A. Alsaif. Morin, a flavonoid prevents lysosomal damage in experimental myocardial ischemic rats, Journal of Medicinal Plants Research, 2012; 6(18): 3445–3449.
6. K¨uhnau J. The flavonoids A class of semi-essential food components: their role in human nutrition, World Review of Nutrition and Dietetics, 1976; 24: 117–191.
7. Shohreh N, Mehrdad H, Mehdi R, Heidar Ali Tajmir-Riahi. DNA Adducts with Antioxidant Flavonoids:Morin, Apigenin, and Naringin, D.N.A and cell biology,2008;27:433-442.
8. Kapoor R, Kakkar P. Protective Role of Morin, a Flavonoid, against High Glucose Induced Oxidative Stress Mediated Apoptosis in Primary Rat Hepatocytes, Plos one,2012; 7(8): 1-11.
9. Caselli A, Cirri P, Santi A, Paoli P. Morin: A Promising Natural Drug,Current Medicinal Chemistry, 2016; 23:1-18.
10. Sonam and Sahu AN. Development and Characterization Of Hepatoprotective Phytosomes of Abutilon Indicum and Piper Longum, International Journal of Pharmacy and Biological Sciences, 2015; 5(4): 97-106.
11. Sikarwar M.S, Sharma.S,Jain A. Preparation characterization and evaluation of marsupsin-phospholipid complex, AAPS Pharm sci Tech,2008; 2(3) :129-137.
12. Jain PK, Khurana N, Pounikar Y, Gajbhiye A, Kharya MD. Enhancement of absorption and hepatoprotective potential through soya-phosphatidylcholine-andrographolide vesicular system, J Liposome Res, 2013 ;1(9):1532-2394.
13. Patel P, Rakesh Baria H,Ashok. Formulation and evaluation of liposomes of ketoconazole, International journal of Drug Delivery Tech , 2009;1(1):16-23.
14. Asija Sangeeta ,Jeyabalan G,Ahuja Anil, Asija Rajesh.Formulation and evaluation of prosopis cineraria (Linn.) druce phospholipid complex,Asian journal of chemical and pharmaceutical research, 2015;3(2):301-305.
15. Jain PK, Kharya murlindhar, Gajbhiye Asmita. Pharmacological evaluation of mangiferin herbosomes for antioxidant and hepatoprotective potential against ethanol induced hepatic damage,Drug development and industrial pharmacy, 2012; 4(6):1-11.
16. Parthasarathi G, Udupa N ,Umadevi P,Pillai G.K. Formulation and in vitro evaluation of vincristrine encapsulsated Niosomes,Journal of Drug Targeting, 1994; 2:173-182.
Statistics
105 Views | 134 Downloads
How to Cite
Manral, K., Singh, A. K., & Sah, V. (2019). Development and Characterization of Morin Loaded Phytosomes for its Anti-Oxidant Activity. Journal of Drug Delivery and Therapeutics, 9(4), 30-36. https://doi.org/10.22270/jddt.v9i4.2971