Development and Validation of UV- Spectrophotometric Method for estimation of Vancomycin Hydrochloride

  • Saikat Pande A. R. College of Pharmacy & G. H. Patel Institute of Pharmacy, Vallabh Vidyanagar, Anand
  • Jolly R Parikh A. R. College of Pharmacy & G. H. Patel Institute of Pharmacy, Vallabh Vidyanagar, Anand, Gujarat, India

Abstract

UV-spectrophotometry refers to absorption spectroscopy or reflectance spectroscopy in the ultraviolet-visible spectral region. This method of analysis is gaining importance as it is rapid, simple, precise, less time consuming and highly accurate. The objective of the present investigation was to develop an accurate, rapid and robust method for determination of Vancomycin hydrochloride in pharmaceutical preparations by using UV spectrophotometric method. Vancomycin hydrochloride shows maximum absorbance at a wavelength of 281 nm, which is used for this study. The method provides a linear response from a quantitation range of 20 µg/ml to 100 µg/ml in phosphate buffer pH 6.8 with regression equation y = 0.0038x + 0.0045 and r2 0.9994. Interday precision and accuracy was found to be below 0.2 and above 99.00% respectively for the developed method. Thus the developed method may be suitably applied for regular quality control of Vancomycin hydrochloride in bulk and pharmaceutical preparations.


Keywords: Vancomycin hydrochloride, spectroscopic method, validation.

Downloads

Download data is not yet available.

Author Biographies

Saikat Pande, A. R. College of Pharmacy & G. H. Patel Institute of Pharmacy, Vallabh Vidyanagar, Anand

A.R. College of Pharmacy & G. H. Patel Institute of Pharmacy, Vallabh Vidyanagar, Anand, Gujarat, India

Jolly R Parikh, A. R. College of Pharmacy & G. H. Patel Institute of Pharmacy, Vallabh Vidyanagar, Anand, Gujarat, India

A.R. College of Pharmacy & G. H. Patel Institute of Pharmacy, Vallabh Vidyanagar, Anand, Gujarat, India

References

1. Barna JC, Williams DH. The structure and mode of action of glycopeptide antibiotics of the vancomycin group. Am Rev microbial, 1984; 38:339-357
2. United States Pharmacopeia and National Formulary (USP 34-NF 29). Vol 3. Rockville, MD: United States Pharmacopeia Convention; 2011. P. 4564-4566.
3. Indian Pharmacopoeia. Vol. 2, Government of India, ministry of Health and Family Welfare, Controller of publications. New Delhi; 2014. P. 1655-1657.
4. Kirby WMM. Vancomycin therapy of severe staphylococcal infections. J Antimicrobial Chemotherapy, 1984; 14 (Suppl D):73-78.
5. Pauly DJ, Musa DM et al. Risk of nephrotoxicity with combination vancomycin-aminoglycoside antibiotic therapy. Pharmaco-therapy 1990; 10:378-382.
6. Dubey SK, Kumar S, Mudakavi RJ, Deshpande S, Jain AK. Development and validation of UV-Spectrophotometric method for determination of Enalapril maleate. International Journal of Advances in Pharmaceutical Sciences., 2010; 1:375-380.
7. Brown EP, Weiner JA, Lin S, et al. Optimization and qualification of an Fc Array assay for assessments of antibodies against HIV-1/SIV. Journal of Immunological Methods. 2018; 455:24-33.
8. Srivastava MM: High Performance Thin Layer Chromatography (HPTLC). Springer, First Edition 2011.
9. Merodio M, Campanero MA, Mirshahi T, Mirshahi M, Irache JM. Development of a sensitive method for the determination of ganciclovir by reversed-phase high-performance liquid chromatography. Journal of Chromatography A. 2000; 870:159-167.
10. Bolton S, Swarbrick J: Basic definition and concept In: Pharmaceutical statistics: practical and clinical and application. Marcel Dekker, New York, Third Edition 1990.
11. Pryseley A, Mintiens K, Knapen K et al. Estimating precision, repeatability, and reproducibility from Gaussian and non-Gaussian data: A mixed models approach. Journal of Applied Statistics, 2010; 37(10):1729-1747.
Statistics
40 Views | 91 Downloads
How to Cite
Pande, S., & Parikh, J. (2019). Development and Validation of UV- Spectrophotometric Method for estimation of Vancomycin Hydrochloride. Journal of Drug Delivery and Therapeutics, 9(3-s), 116-118. https://doi.org/10.22270/jddt.v9i3-s.2959