Simultaneous Estimation & Validation of Praziquantel & Pyrantel Pamoate in Bulk & Pharmaceutical Dosage Form by Using RP-HPLC
The first reversed phase high performance liquid chromatographic method for quantitation studies of, Praziquantel and Pyrantel Pamoate has been developed and validated to be a simple, sensitive, rapid, specific, precise, and accurate method. Chromatographic separation was achieved on C18 column (250×4.6 mm-5μm p.s). Methanol and water in a ratio [85:15 v/v] as a mobile phase at flow rate of 0.8ml/min. UV detection was operated at 217 nm and injection volum was 20 μl.. The proposed method showed good linearity, accuracy, precision and was successfully applied for determination of the drugs in laboratory prepared pharmaceutical dosage forms. The current method has been statistically validated according to the ICH guidelines and this method has been subsequently developed and applied successfully to determine the levels of Praziquantel and Pyrantel pamoate in a combined formulation and in the routine quality control analysis with good accuracy and sensitivity.
Keywords: Praziquantel, Pyrantel pamoate, Quantitation Studies, RP-HPLC ,
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