Protective role of Carica papaya and Ficus bengalensis latex against CCl4 induced liver toxicity in experimental rats
Objective: To study the hepatoprotective activity of Carica Papaya Latex at the dose 400mg/kg/b.w. per oral and Ficus Bengalensis latex at dose 300mg/kg/b.w. per oral against CCl4 induce Hepatotoxicity in albino rats.
Method: Animals were divided into 5 groups of 6 animals in each group.
Group I – Served as a normal control received saline 1ml /Kg for 21 days. The group -II, III, IV and V animals were received CCl4 at dose 1ml/Kg b.w./day intra- peritoneally. Animals from group –III to V received Carica Papaya Latex (400mg/Kg.p.o.), Ficus Bengalensis Latex (300mg/Kg.p.o.) and standard drug Silymarin (30mg/Kg.p.o.) once daily for 21 days. All groups animals were sacrificed on 22 days under light ether anesthesia . Blood sample were collected and used for bio-chemical parameters like serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), Bilirubin(BRN), Alkaline phosphate (ALP) and total protein.
Result:The results were observed that Carica Papaya latex at dose 400 mg/kg.b.w and CCl4 treated group , Ficus Bengalensis latex at dose 300 mg/kg.B.w and CCl4 treated groups were found the decreased levels of SGPT,SGOT,, ALP and Bilirubin and increased total protein level compare to CCl4 treated group .So that this study indicated hepatoprotective effect .
Conclusion: Present investigation indicated that both latex of Carica papaya and Ficus Bengalensis showed significant protection against CCl4 nduced hepatotoxicity in Rats.
Keywords: Carica Papaya, Ficus Bengalensis, Hepatoprotective, Carbon Tetra Chloride, Silymarin.
2. Wolf PL. Biochemical diagnosis of liver diseases. Indian Journal of Clinical Biochemistry 1999; 14:59-90.
3. Joseph B, Justin Raj S, An overview – Ficus Bengalensis Linn, International Journal of Pharmaceutical Science review ad research, 2011; 6(1):21-24.
4. Arya VS, Indian medicinal plants: A compendium of 500 species. Universe press, volume 3, pp 20-21.
5. Chatarjee A, the treaties of Indian Medicinl Plants : Vol.I, 1997, pp.39.
6. Augusti KT, Hypoglycemic action of bengalenoside : Aglucoside isolated from Ficus Bengalensis Linn, in normal and Alloxan diabetic rabbits. Indian J Physiol Pharmacol: 1975; 19:218-220.
7. Augusti KT, Daniel RS, Cherian S, Sheela CG. Effect of Leucopelargonin derivative derivative from Ficus Bengalensis Linn on diabetic dogs Indian J Med Res: 1994; 82-86.
8. Kumar RV, Augusti KT, Insulin sparing action of a leucocynidin derivative isolated from bengalensis Linn Indian Journal of Biochemistry and Biophysics: 1994; 31:73-76.
9. Patil VV, Pimprikar RB, Patil VR, Pharmacognostical studies and evaluation of anti-inflammatory activity of Ficus Bengalensis Linn, Pharmacognosy 2009; 1(1):49-53.
10. Shukla R, Gupta S, Gambhir JK, Prabhu KM. Antioxidant effect of aqueous extract of bark of ficus bengalensis in hypercholesterolaemic rabbits J Ethnopharmacol, 2004; 92 (1):47-50.
11. Mikhalchik EV lvanova AV., Anurov MV., wound healing effect of papaya based preparation in experimental thermal trauma. Bulletin of experimental biology and medicinal. 2004; 137:560-562.
12. Monti, R., Contiero J., Goulart AJ., Isolation of natural inhibitors of papain obtained from Carica Papaya latex. Brazilian Archives of Biology and technology, 2004; 47:747-754.
13. Arya VS, Indian medicinal plants: A compendium of 500 species. Universe press, volume 1, pp 383-385.
14. Emeruwa AC. Journal of Natural Product 1982; 45(2):123-127.
15. Chinoy NJ, Patel KG, Sunita C, J Med Arom Plant SCi. 1997; 19(2):422-426.
16. Gurung S, Skalko- Basnet N. Wound healing properties of Carica Papaya Latex: In vivo evaluation in mce burn model, Journal of Ethanopharmacology, 2009; 121:338-341.
17. Geetha BS, Mathew BC, Augusti KT, Hypoglycimic effects Leucodelphinidin derivative isolated from Ficus Bengalensis, Indian Journal Physiol Pharmacol, 1994; 38:220-2.
18. Trease G.E. and Evans W.C. , Pharmacognosy. 11th Edition, Bailliere Tindall, London, 1989; 45-50.
19. Veeraraghavan P, Expert consultant, CPCSEA, OECD guideline Nol. 420.
20. Suresh Kumar SV, Mishra SH. Hepatoprotective effect of Perguleria daemia (Forsk.) ethanol extract and its fraction. Indian Journal of Experimental Biology, June 2008; 46:447-452.
21. Shanthasheela R., Chitra M., and Vijayachitra R., Evaluation of Hepatoprotective activity of combination of anethum graveolens and agave Americana on CCl4 intoxicated rats. Indian drugs, 2007; 44(12):950-952.
22. Manjunatha BK, Hepatoprotective activity of Pterocarpus Santalinus L.F,an endangered medicinal plant.Indian J Pharmacol, 2006; 38(1).
23. Recknagel R.D., Glende E.A., Jr. Dolak and walter R.L. Mechanism of Carbon Tetra Chloride toxicity pharmacol ther, 1989; 43:139-54.
24. Protman R. B. and Lawhorn G.T., Serum enzymes are indicators of chemical induced liver damage, drug chem. Toxicol, 1978; 1: 163.
25. Wolf PL., Biochemical diagnosis of liver disease. Indian Journal Clin biochem, 1999; 14: 59-65.
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