Inhibition of Innate Immune Responses of Polymorphonuclear Leucocytes and Monocytes by Rhinacanthin‑C
Phagocytosis is a pivotal microbicidal function of innate immune cells by which phagocytes would engulf and eliminate invading pathogens. The functioning and efficacy of immune system is altered by many pathogens e.g. bacteria and fungi and the nonspecific kind of innate immunity is responsible for providing protection against these invading pathogens. The aim of this study is to assess the inhibitory effect of rhinacanthin‑C on phagocytic activities to obtain important insights into its ability to suppress phagocytes. Phagocytosis activity of innate immune cell was monitored using a flowcytometer and myeloperoxidase activity assay was conducted using a myeloperoxidase activity colorimetric assay. Rhinacanthin‑C at 100.0 and 6.25 μg/ml significantly showed strong inhibition of phagocytosis with percentage of 26.40% and 30.59% respectively, in comparison with the positive control (p< 0.001). A dose-dependent (50.0, 25.0, 12.5, 6.25 and 3.13 μg/ml) inhibition of MPO activity of rhinacanthin-C was observed and show high activity compared to control cells (p< 0.001). This finding indicated that rhinacanthin-C was able to suppress human phagocyte response and emphasizing their potency as immunomodulatory agents. Nevertheless, further investigations are needed to elucidate other immunomodulatory responses.
Keywords: macrophage, monocyte, myeloperoxidase, phagocytosis, rhinacanthin-C
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