Pharmacological study of trunk bark of Acacia nilotica var adansonii (Guill et Perr).o Ktze (Mimosaceae): Assays, antioxidant and antispasmodic activities

  • Gilchrist L Boly Abdoul
  • Aristide Traore
  • Moussa Ouedraogo
  • Mohamed Belemlilga
  • Tata K. Traore
  • Lazare Belemnaba
  • Noufou. Ouedraogo
  • Andre Lupu
  • Sylvin Ouedraogo
  • Mirn Liviu o
  • Innocent P Guissou


Aim of this study was to evaluate in vitro polyphenols content, antioxidant and antispasmodic properties of the aqueous extract and fractions of the trunk bark of Acacia nilotica. According to a survey conducted in rural Burkina Faso, Acacia nilotica var. adansonii (Guill and Perr). Ktze reported to be widely used in the treatment of gastrointestinal diarrhoea and parasitosis. A maceration of the powder of the trunk bark of the plant was carried out. Then the aqueous macerate obtain, was fractionated with dichloromethane, butanol and ethyl acetate successively. The phenolic compounds of the aqueous extract, butanol and ethyl acetate fractions was determinated. The antioxidant activity of aqueous extract and fractions was evaluated by the DPPH, ABTS and FRAP tests. The contractility test on smooth muscle was realized according to Magnus method. Assay of the extracts revealed a high content of polyphenols, tannins and flavonoids. The aqueous extract, the butanol fraction and the ethyl acetate fraction demonstrated a high antioxidant capacity. Aqueous extract showed a better antispasmodic effect of acetylcholine contraction induction at 1 μM (IC50 = 13.02 μg / mL) and for BaCl2 at 160 μg / mL (IC50 = 117.2 μg / mL). The aqueous extract of Acacia nilotica and his fractions had antioxidant properties. Only aqueous extract proven better antispasmodic property. Hence its use in traditional medicine in the treatment of diarrhoea.

Keywords: Acacia nilotica, Antioxidant, Antispasmodic


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1. Nacoulma O., Plantes médicinales et pratiques médicales traditionnelles au Burkina Faso : cas du plateau central. Thèse de Doctorat ès Sciences Naturelles, Faculté des Sciences et Techniques de l'Université de Ouagadougou, 1996 ; 320 p.
2. Traore A., Ouedraogo Sylvin., Lompo M., Traore S., Some N., Guissou I.P., Ethnobotanical survey of medicinal plants used to treat gastrointestinal parasites in human and livestock in four geographic areas of Burkina Faso (West Africa), Scholars Research Library, 2013; 5 (6):172-177.
3. Boly A.G.L., Belemlilga M., Traoré A., Ouédraogo S., Guissou I.P., Phytochemical and in vitro anthelminthic properties studies of Acacia nilotica var adansonii (Guill & Perr). O Ktze (MIMOSACEAE) trunk barks aqueous extract, International journal of pharmacognosy and phytotherapy research, 2018;10 (1): 5-10
4. Saini M.L, Comparative Pharmacognostical and antimicrobial studies of Acacia species (Mimosaceae). Journal of Medicinal Plants Research., 2008; 2(12):378-386.
5. Magnus In., Pharmacological experiments on isolated preparations Churchill Linvingstone. « Staff of the departement of pharmacology », University of Edinbourgh, 1970; 62p.
6. Guinko S, Etude sur le rôle des Acacia dans le développement rural au Burkina Faso et au Niger, IRD Editions Ouagadougou,1991; 36p.
7. Singleton V. L., Orthofer R., Lamuela-Raventos R. M, Analysis of phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalceu Reagent, Methods in Enzymology, 1999; 299: 152-178.
8. Tibiri. A., Rakotonandrasana. O., NACOULMA. G. O., BANZOUZI. J. T, Radical Scavenging Activity, Phenolic Content and Cytotoxicity of Entada africana Guill. Et Perr. (Mimosaceae), Journal of Biological Sciences, 2007; 7(6): 959-963
9. Abdel Hameed, El-Sayed Saleh, Total phenolic contents and free radical scavenging activity of certain Egyptian Ficus species leaf samples. Food Chemistry, 2008; 114(4):1271-1277.
10. Kim, K.S., Lee, S., Lee, Y.S., Yung, S.H, Park, Y., Shin, Kh., Kim, B-K, Anti-oxidant activities of the extracts from the herbs of Artemisia apiacea, J.Ethnopharmacol., 2003; 85: 69-72.
11. Re R., Pellegrini N., Proteggente A., Pannala A., Yang M., Rice-Evans C, Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radical Biology and Medicine, 1999; 26(9-10):1231-1237.
12. Apati P., Szentmihalyi K., Kristo S. T., Papp I., Vinkler P., Szoke E., Kery A, Herbal remedies of Solidago correlation of phytochemical characteristics and antioxidative properties, Journal of Pharmaceutical and Biomedical Analysis, 2003; 32(4-5): 1045-1053
13. Mukundi MJ., Piero NM., Mwaniki NEN., Murugi NJ., Daniel AS., Antidiabetic Effects of Aqueous Leaf Extracts of Acacia nilotica in Alloxan Induced Diabetic Mice, J. Diabetes Metab., 2015; 6: 568.
14. Serémé A., Millogo J., Guinko S., Nacro M, Concentration à tanins des organes de plantes tannifères du Burkina Faso, J. Soc. Ouest-afriq. Chim, 2008; 025: 55-61.
15. B Singh., Sukhpreet Singh., Neeraj Kumar., Subodh Kumar., Saroj Arora, Umbelliferone – An antioxidant isolated from Acacia nilotica (L.) Willd. Ex. Del. Rajbir, Food Chemistry, 2010; 120 : 825–830.
16. Omara A., Somaia A., Nada Abdel Razik., Farrag H., Walid M, Therapeutic effect of Acacia nilotica pods extract on streptozotocin induced diabetic nephropathy in rat, Phytomedicine, 2012; 19: 1059- 1067.
17. Sadiq M., Hanpithakpong W., Tarningb J., Kumar A Anal, Screening of phytochemicals and in vitro evaluation of antibacterial and antioxidant activities of leaves, pods and bark extracts of Acacia nilotica (L.) Del. Industrial Crops and Products, 2015; 77: 873–882.
18. Patil A. P., Patil V. V., et Patil V. R. 2009, In-vitro free radicals scavenging activity of Madhuca indica Gme, Pharmacologyonline, 2009; 2:1344-1352
19. Barapatre A., Singh A. M., Mekala S., Das A, Harit Jha, In vitro evaluation of antioxidant and cytotoxic activities of lignin fractions extracted from Acacia nilotica, International Journal of Biological Macromolecules. 2016; 01:109-115
20. Bangou M J, Etude phytochimique et activités biologiques des tiges feuillées de Lantana camara L. et de Lippia chevalieri Moldenke: deux VERBENACEAE du BURKINA FASO, Thèse unique en Biochimie et Chimie des Substances Naturelles Université de Ouagadougou (Burkina Faso), 2012 ; 199p
21. Duh PD., Tu YY, Yen GC, Antioxidant activity of the aqueous extract of harn jyur (Chrysanthemum morifolium Ramat). Lebensmittel-Wissenschaft and Technologie 1999; 32: 269-277.
22. Gordon MH, The mechanism of antioxidant action in vitro. In: BJF Hudson (Ed.), Food antioxidants Elsevier Applied Science, London, 1990; 1–18.
23. Surveswaran S., Cai Y-Z., Corke H., Sun M, Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants. Food Chemistry, 2007 ;102: 938-953
24. Meda, N-T.R. Etude phytochimique et activités biologiques des galles et des feuilles de Balanites aegyptiaca (L.) Del. (Balanitaceae) utilisées en médecine traditionnelle au Burkina Faso. Thèse Unique, Université de Ouagadougou, 2010 ; 183p.
25. Awah F.M., Uzoegwu P.N., Oyugi J.O., Rutherford J., Ifeonu P, Eze M.O, Free radical scavenging activity and immunomodulatory effect of Stachytarpheta angustifolia leaf extract. Food Chemistry, 2010; 119: 1409 – 1416
26. Kamalraj R and Devdass, Antispasmodic studies on leaf extract of Erythrina indica Lam. International Journal of Research in Ayurveda & Pharmacy, 2011; 2(4): 1380-1382
27. Thaina P., Poonpanang P., Kitja Sawangjaroen, Comparison of Spasmolytic Activities of Piper longum, P. sarmentosum and Quercus infectoria Extracts with Loperamide and Verapamil in Rat and Guinea Pig Intestinal Tissues, In : III WOCMAP Congress on Medicinal and Aromatic Plants, Traditional Medicine and Nutraceuticals 680, 2003; 6:183-189.
28. Dixit A., P Tiwari., D Parasher., S Bhargava (2014): Antispasmodic studies on leaf and root extract of Blumea lacera. International Journal of Pharmacy and Pharmaceutical Sciences. Vol 6, Issue 1.
29. Niaz A., Wadood S., Shah A, Antispasmodic activity of Teucrium stocksianum boiss, Pakistan journal of pharmaceutical sciences. 2014; 24 (2) : 171-174.
30. Zhu F, Huang B, Hu CY, Jiang QY, Lu ZG, Lu M, Wang MH, Gong M, Qiao CP, Chen W, Huang PH, Effects of total flavonoids of Hippophae rhamnoides L. on intracellular free calcium in cultured vascular smooth muscle cells of spontaneously hypertensive rats and Wistar-Kyoto rats. Chin J Integr Med. 2005; 11 (4): 287-292.
31. Ko TF., Weng YM., Lin SB., Chiou RY., Antimutagenicity of supercritical CO2 extracts of Terminalia catappa leaves and cytotoxicity of the extracts to human hepatoma cells. J Agric Food Chem, 2003; 51(12): 3564-3567.
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Boly Abdoul GL, Traore A, Ouedraogo M, Belemlilga M, Traore TK, Belemnaba L, Ouedraogo N, Lupu A, Ouedraogo S, o ML, Guissou IP. Pharmacological study of trunk bark of Acacia nilotica var adansonii (Guill et Perr).o Ktze (Mimosaceae): Assays, antioxidant and antispasmodic activities. JDDT [Internet]. 15Jun.2019 [cited 8Aug.2020];9(3-s):524-30. Available from: