Ethanolic extract of Annona muricata leaf and its effect on the liver
The effect of ethanolic extract of Annona muricata leaf on the liver of albino rats were investigated in this study. Twenty (20) male albino rats weighing between 180-200 g were used in this study. The animals were divided into groups A, B, C and D. Group A was treated with 2 ml/kg body weight of distilled water, Group B was treated with 100 mg/kg body weight of the extract, Group C was treated with 200 mg/kg body weight of the extract and Group D was treated with 300 mg/kg body weight of the extract. The treatments were given orally and lasted for a period of 30 days. After the last day of treatment, the animals were sacrificed and the liver harvested, weighed and fixed in 10% formal saline for histological studies. Blood samples were collected through cardiac puncture for biochemical analysis. Data were analysed using one-way ANOVA and SPSS version 2.0. Results showed a significant (P<0.05) increase in liver weight when compared to control and a significant (P<0.05) increase in serum levels of ALP, ALT and AST at highest dose. Histopathological findings showed distortions of the liver cytoarchitecture with the highest dose having a more significant effect. Consumption of ethanolic extract of A. muricata leaf at higher doses has the potential of causing liver damage. Thus, its consumption should be regulated or better still taken at lower doses.
Keywords: Annona muricata; Liver; Liver enzymes; Albino rats
2. Akah PE, Okolo CE and Ezike AE. The Haematinic activity of the Methanol leaf Extract of Brillantasia nitens Lindau (Acanthaceae) in rats. African Journal of Biotechnology, 2009; 8(10):2389-2393.
3. Misas C, Hernández N, Abraham A. Contribution to the biological evaluation of cuban plants. Rev Cubana Med Trop, 1979; 31: 29-35.
4. Sundarrao K, Burrows I, Kuduk M, Yi D, Chung MH, Suh NJ, Chang MI. Preliminary screening of anti-bacterial and anti-tumor activities of papuan new guinean active medicinal plants. Pharmaceutical Biol, 1993; 31:3-6.
5. Adjanohoun E, de Souza S, et Sinsin B. La biodiversité face au développement des industries pharmaceutiques africaines. In : Réseau des « espèces ligneuses médicinales, 2000.
6. Antoun MD, Gerena L, Milhus WK. Screening of the flora of Puerto Rico for potential antimalarial bioactives. Int J Pharmacol. 1993; 31: 255-8.
7. Thakkar JH, Solanki HK, Tripathi P, Patel NJ, Jani GK. Evaluation of antimutagenic potential of Annona squamosa leaf extract. Elixir Human Physiology. 2011; 31:1960-1965.
8. Heinrich M, Kuhnt M, Wright CW. Parasitological and microbiological evaluation of mixed Indian medicinal plants (Mexico).J Ethnopharmacol, 1992; 36:81-5.
9. Liaw CC, Wu TY, Chang FR,.Historic perspectives on annonaceous acetogenins from the chemical bench to preclinical trials. Planta Med 2010; 76:1390-404.
10. Moghadamtousi SZ, Kadir HA, Paydar M, Rouhollahi E, Karimian H. Annona muricata leaves induced apoptosis in A549 cells through mitochondrial-mediated pathway and involvement of NF-κB.BMC Complement. Altern. Med. 2014; 14:299.
11. Uboh FE, Okon IE and Ekong MB. Effects of Aqueous Extract of Annona muricata leaves on Liver Enzymes, Histological Integrity and Hematological Indices in Rats. Gastroenterology Research, 2010; 3(1):32-38.
12. Li K, Li Q, Li J. Alkaloid from angelicae daharaicae inhibits hela cell growth by inducing apoptosis and increasing cascape–3 activity. Lab Med 2008; 399:540-6.
13. Alvarez CO, Neske A, Chahboune N. Ucupentol, a novel mono-tetrahydrofuranic acetogenin from Annona montana, as a potent inhibitor of mitochondrial complex I. Chem Biodivers 2009;6:335-40.
14. Nascimento GNL, Valadares MC, Nishijo H, Investigation of the toxic potential of crude ethanol extract of Annona coriacea (araticum) seeds in acute exposed mice. Brazilian J Pharmacog 2012; 22:580-6.
15. Nwogu LA, Igwe CU and Emejulu AA. Effects of Landolphia owariensis on the Liver function profile and haemoglobin concentration of albino rats. African Journal of Biochemistry Research, 2010; 2(12):240-242.
16. Owolabi F, William OE and Edeh OJ. Annona muricata nutritional value. Nature, 2013; 10(20):234-237.
17. Ezejindu N, Udemezue O, Chukwujekwu IE, Uchefuna RC, Maduka SO, Akingboye AJ, Ezejindu CN. The histological effect of ethanolic leaf extracts of Annona muricata (soursop) on liver of adult wistar rats. IJHSR 2015; 5:125–129.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeÂ The Effect of Open Access).