Development and Validation of UV-Visible Spectrophotometric method for simultaneous estimation of Etoposide and Picroside-II in bulk and pharmaceutical formulation
Aim: To develop and validate a simple, precise, accurate, and sensitive UV-visible spectrophotometric method for the simultaneous estimation of Etoposide (ETO) and Picroside-II (PK-II) in a bulk and pharmaceutical formulation according to the ICH guidelines.
Methods: The absorption spectra of ETO and PK-II were carried out over the range of 200-800 nm, and absorption maxima were determined. Multiple calibration standards were prepared of both the drugs separately, and absorbance were recorded at respective λmax. Calibration curve were plotted and the linear responses were studied. Various analytical method validation parameters viz. accuracy, precision, LOD, LOQ, robustness and ruggedness were calculated using QC standards.
Results: The absorption maxima of ETO and PK-II were found to be 208 nm and 265 nm respectively. Linearity range for ETO and PK-II were found to be 1-7 μg/ml and 1-35 μg/ml with correlation coefficient 0.999 and 0.999. The intra-day and inter-day study shows percent relative standard deviation in between 0.11 to 1.08 and 0.12 to 1.38. LOD and LOQ were found to be 0.1321 μg/ml and 0.4003 μg/ml for ETO whereas 0.1616μg/ml and 0.4897μg/ml for PK-II. The total percent recovery of ETO and PK-II were found to be 99.09 and 99.68 respectively.
Conclusion: The simple, precise, accurate, and sensitive UV-visible spectrophotometric method for the simultaneous estimation of Etoposide (ETO) and Picroside-II (PK-II) in a bulk and pharmaceutical formulation was developed and validated.
Keywords: UV-visible spectrophotometry, simultaneous estimation, Etoposide and Picroside-II.
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