Free Radical Scavenging Potential of Mentha arvensis of South Gujarat: Evidence from In-vitro Assay

  • Amol R Kharat, Dr Government College of Pharmacy, Ratnagiri, India-415612
  • Farooqui Zakerab
  • Kiran Ramesh Kharat Centre For Advanced Life Sciences , Deogiri college ,Aurangabad


Present work was undertaken to evaluate efficacy of aqueous and methanol extracts Mentha arvensis leaves as free radical scavenger. The free radical scavenging activity was evaluated by nitric oxide scavenging method, hydrogen peroxide scavenging method and ferric thiocyanate method. The result of the studies was compared with the standard solution of Ascorbic Acid (Vitamin C) treated with same reagent. The results of all the studies showed significant free radical scavenging activity in both aqueous and methanolic extracts of Mentha arvensis and methanolic extract showed the highest free radical scavenging potential than aqueous extract.

Keywords: Free radical, Mentha arvensis, Nitric oxide, Hydrogen peroxide, ferric thiocyanate


Download data is not yet available.


1. Hassan BAR, Medicinal plants importance and uses. Pharmaceutica Analytica Acta, 2012; 3(10):1000-1139.
2. Mahdi-Pour B, Jothy SL, Latha LY, Chen Y, Sasidharan S, Antioxidant activity of methanol extracts of different parts of Lantana camara, Asian Pacific Journal of Tropical Biomedicine, 2012; 2(12):960–965.
3. Kanwar JR, Kanwar RK, Burrow H, Baratchi S, Recent advances on the roles of NO in cancer and chronic inflammatory disorders, Current Medicinal Chemistry, 2009; 16:2373–2394.
4. Chiavaroli V, Giannini C, De Marco S, Chiarelli F, Mohn A, Unbalanced oxidant-antioxidant status and its effects in pediatric diseases, Redox Report, 2011; 16:101–107.
5. Yamagishi S, Matsui T, Nitric oxide, a Janus-faced therapeutic target for diabetic microangiopathy-Friend or foe?, Pharmacological Research, 2011; 64:187–194.
6. Wu YY, Li W, Xu Y, Jin EH, Tu YY, Evaluation of the antioxidant effects of four main theaflavin derivatives through chemiluminescence and DNA damage analyses, Journal of Zhejiang University Science B, 2011; 12:744–751.
7. Thawkar BS, Jawarkar AG, Kalamkar PV, Pawar KP, Kale MK, Phytochemical and pharmacological review of Mentha arvensis, 2016; 10(2):76.
8. Kapoor LD. Handbook of Ayurvedic Medicinal Plants. 1st ed. Boca Raton, FL: CRC Publication; 2000. p. 227-8.
9. CSIR. Wealth of India, Raw Materials. Vol. 43. New Delhi, India: Council of Scientific and Industrial Research; 1972. p. 337-46.
10. Coutinho HD, Costa JG, Lima EO, Falcao-Silva VS, Siqueira-Júnior JP, Potentiating effect of Mentha arvensis and chlorpromazine in the resistance to aminoglycosides of methicillin-resistant Staphylococcus aureus. In vivo, 2009; 23(2):287-9.
11. Kowti R, Vishwanath S, Shivakumar SI, Vedamurthy J, Abdul NK, Hepatoprotective and Antioxidant Activity of Ethanol Extract of Mentha arvensis Leaves Against Carbon Tetrachloride Induced Hepatic Damage In Rats, International Journal of PharmTech Research, 2013; 5(2):426-430.
12. Kanjanapothi D, Smitasiri Y, Pathong A, Taesotikul T, Rathanapanone V,Postcoital antifertility effect of Mentha arvensis, Contraception, 1981; 24:559-567.
13. Verma SM, Arora H, Dubey R, Anti–Inflammatory and Sedative –Hypnotic Activity of the methanolic extract of the leaves of Mentha Arvensis, Ancient Sci Life, 2003; 23(2):95–99.
14. Marta C, Teixeira D, Glyn MF, Anti-candida activity of Brazilian medicinal plants, Journal of Ethanopharmacology, 2005; 97:305-311.
15. Ganesh CJ, Manjeshwar SB, Influence of the leaf extract of Mentha arvensis Linn. (mint) on the survival of Mice exposed to different doses of Gamma Radiation. strahlentherapie onkologie, 2002; 178:91-8.
16. Kokate CK, Practical Pharmacognosy, 1st ed.,VallabhPrakashan, New Delhi, 1986 b, 111.
17. Josiah BH, Bartholomew OI, In vitro antioxidant capacity and free radical scavenging evaluation of active metabolite constituents of Newbouldia laevis ethanolic leaf extract, Biological Research, 2015; 48(1):16
18. Rozina P, Kundu SK, Saha P, In Vitro Nitric Oxide Scavenging Activity Of Methanol Extracts of Three Bangladeshi Medicinal Plants, 2013; 1(12):83-88.
19. Ahmed D, Khan MM, Saeed R, Comparative Analysis of Phenolics, Flavonoids, and Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum caudatum Leaves, Antioxidants, 2015; 4:394-409.
20. Ruch RJ, Cheng SJ, Klaunig JE, Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea, Carcinogenesis, 1989; 10:1003-1008.
21. Kikuzaki H, Nakatani N, Antioxidant effect of some ginger constituents. Journal of Food Science, 1993; 58(6):1407–1410.
22. Ferreira ICFR, Baptista P, Vilas-Boas M, Barros L, Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: individual cap and stipe activity, Food Chemistry, 2007;100(4):1511–1516.
23. Panda P, Aiko V, Mehta A, Effect of aqueous extracts of Mentha arvensis (mint) and Piper betle (betel) on growth and citrinin production from toxigenic Penicillium citrinum, Journal of Food Science Technology, 2015; 52(6):3466–3474.
103 Views | 161 Downloads
How to Cite
Kharat A, Zakerab F, Kharat K. Free Radical Scavenging Potential of Mentha arvensis of South Gujarat: Evidence from In-vitro Assay. JDDT [Internet]. 15May2019 [cited 14Apr.2021];9(3):263-8. Available from: