Pharmacognostic Investigation and HPTLC Fingerprinting of a Siddha Polyherbal Drug, Padai chankaran

  • B Neethu Kannan
  • V Gayathri Devi
  • John Anitha
  • GS Lekha
  • M Natarajan
  • A Kanagarajan


The present study aims to establish the quality and purity of a Siddha formulation, Padai chankaran by laying down various pharmacognostic parameters, physico-chemical constants and HPTLC fingerprint profiles. Padai chankaran is a Siddha polyherbal preparation comprised of Catunaregum spinosa – root bark, C. spinosa – seed and Alangium salvifolium – root bark as the ingredients. The formulation is used as an external application, having astringent, anthelmintic and antiseptic activities that supports in healing of ulcers and dermatological diseases. Powder microscopy studies and physico-chemical analysis were carried out. Also, an attempt has been made to develop a HPTLC method for phytochemical fingerprinting and the mobile phase Toluene: Ethyl acetate: Formic acid (5: 2: 0.1) gave the best resolution for various components. Hence, the aforesaid analyses confirmed the purity and quality of the Siddha formulation for their future applications.

Keywords: Padai chankaran, powder microscopy, physico-chemical, HPTLC studies


Download data is not yet available.


1. Khushbu C, Roshni S, Anar P, Carol M, Mayuree P. Phytochemical and therapeutic potential of Piper longum Linn a review. International Journal of Research in Ayurveda and Pharmacy. 2011; 2(1):157-61.
2. Meena AK, Rao MM, Panda P, Kiran YA, Singh Uttam SB. Standardisation of ayurvedic polyherbal formulation, Pancasama Churna. International Journal of Pharmacognosy and Phytochemical Research. 2010; 2(1):11-4.
3. Muthaliyar M. Gunapadam Mooligai Vaguppu – Siddha Materia Medica (Medicinal Plants division), fifth edition 1998; Directorate of Indian Medicine and Homeopathy; Page 52-53, 730-31.
4. Trease GE, Evans WC. Pharmacognosy. 2002; 15th ed. London: WB Saunders.
5. Kadam PV, Yadav KN, Deoda RS, Narappanawar NS, Shivatare RS, Patil MJ. Pharmacognostic and phytochemical studies on roots of Agave Americana (Agavaceae). International Journal of Pharmacognosy and Phytochemical Research. 2012; 4(3):92-6.
6. Rakholiya K, Kaneria M, Chanda S. Physicochemical and phytochemical analysis of different parts of Indian Kesar mango–a unique variety from Saurashtra region of Gujarat. Pharmacognosy Journal. 2016; 8(5): 502-506.
7. Thomas S, Patil DA, Patil AG, Chandra N. Pharmacognostic evaluation and physicochemical analysis of Averrhoa carambola L. fruit. Journal of Herbal Medicine and Toxicology. 2008; 2(2):51-4.
8. Patel R, Mahobia N, Waseem N, Upwar N, Singh S. Phyto-physicochemical investigation of leaves of Plectranthus amboinicus (Lour) Spreng. Pharmacognosy Journal. 2010; 2(13):536-42.
9. Ahmed S, Hasan MM. Standardization of crude drugs: a precise review. World Journal of Pharmaceutical Research. 2015; 4(10):155-74.
10. Keerthana K, Jothi G, Gurunagarajan S, Nayagam AA. Microscopic, physicochemical and phytochemical studies of Gardenia jasminoides. [Ellis]. International Journal of Pharmacy and Pharmaceutical Sciences. 2018; 10:97-102.
11. Jothi G, Keerthana K & Sridharan G. Pharmacognostic, physicochemical, and phytochemical studies on stem bark of Zanthoxylum armatum DC. Asian Journal of Pharmaceutical and Clinical Research. 2019; 12(2):1-5.
12. Kulkarni YA, Gokhale SB, Yele SU, Surana SJ, Tatiya AU. Pharmacognostical studies and preliminary phytochemical investigations on the bark of Persea macrantha (Nees) Kosterm (Lauraceae). Indian Journal of Natural Products and Resources. 2011; 2(2):211-217.
13. Neethu KB, Gayathri Devi V, Anitha John Lekha GS, Natarajan M, Kanagarajan A. Pharmacognostic evaluation and HPTLC fingerprinting of Pirantai Vatakam, a Siddha formulation. The Journal of Phytopharmacology. 2019; 8(2):33-37.
14. Tambe R, Singhal RG, Bhise K, Kulkarni M. Phytochemical screening and HPTLC fingerprinting of leaf extracts of Psidium guajava Linn. Journal of Pharmacognosy and Phytochemistry. 2014; 3(1):52-56.
15. Murugesan S, Bhuvaneswari S, Sivamurugan V. Phytochemical screening and HPTLC fingerprint profile of marine red alga Spyridia fusiformis Boergesen. Haya: The Saudi Journal of Life Sciences. 2016; 1(4): 124-129.
38 Views | 77 Downloads
How to Cite
Neethu Kannan, B., Gayathri Devi, V., Anitha, J., Lekha, G., Natarajan, M., & Kanagarajan, A. (2019). Pharmacognostic Investigation and HPTLC Fingerprinting of a Siddha Polyherbal Drug, Padai chankaran. Journal of Drug Delivery and Therapeutics, 9(3-s), 317-323.