Isolation and Screening of Actinomycetes producing Antibacterial compounds from different river sediments

  • Sahil Abbas Department of Pharmacy, Bhagwant University, ajmer, Raj., India
  • Girendra Gautam Department of Pharmacy, Bhagwant University, ajmer, Raj., India
  • Pawan Kumar Gautam Department of Pharmacy, Sarojini naidu medical college, Agra, U.P., India

Abstract

The history of antibacterial begins with the observations of Pasteur and Joubert, who discovered that one type of bacteria could prevent the growth of another. They did not know at that time that the reason one bacterium failed to grow was that the other bacterium was producing an antibiotic. Of course, in today's common usage, the term antibiotic is used to refer to almost any drug that attempts to rid your body of a bacterial infection. The past researches indicated that huge number of antibiotics was produced by Gram +ve ike bacteria known as Actinomycetes. So we can say that among all microbes more than 50% of the known antimicrobial compounds were produced by Actinomycetes only.  In our study isolation and screening of Actinomycetes was performed by using different river sediments. Soil samples was collected from river Godaveri and Krishna and   stored in the U.V. and alcohol sterilized Poly bags. Soil samples was serially diluted up to 10-6 and 1 ml from each dilution was plated on different isolation media like starch Casein agar, Albumin media and YMA media, consisting of antifungal agent Nystatin 50 µg/ ml, by pour plate technique. The plates were incubated at different temperature ranges 18 0C to 28 0C upto 7-14 days. Determination of antibacterial activities of pure actinomycetes cultures of S1, S2, S3 and S4 were performed by using streak -plate method. Mueller hinton agar plates will prepared and inoculated with actinomycetes cultures by a single streak of inoculums in the center of the Petri dish and will incubated at 270C for 4 days. Later, the plates will seeded with test organisms by a single streak at a 900 angle to actinomycetes strains. Antagonism was measured by the determination of the size of the inhibition zone. The antibacterial activity of compound was   tested against different gram +ve and Gram –ve by the standard disc diffusion method and cup plate method. Standard streptomycin was used for comparison of the antibacterial activity.

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Author Biographies

Sahil Abbas, Department of Pharmacy, Bhagwant University, ajmer, Raj., India

Department of Pharmacy, Bhagwant University, ajmer, Raj., India

Girendra Gautam, Department of Pharmacy, Bhagwant University, ajmer, Raj., India

Department of Pharmacy, Bhagwant University, ajmer, Raj., India

Pawan Kumar Gautam, Department of Pharmacy, Sarojini naidu medical college, Agra, U.P., India

Department of Pharmacy, Sarojini naidu medical college, Agra, U.P., India

References

1. Librado OO, Luis FB, Veronica Y. Biological, Biochemical, and Biomedical Aspects of Actinomycetes. Academic Press New York; 1984 : 453.
2. Hung PQ, Kumar SM, Govindsamy V and Annapurna K. Isolation and characterization of Endophytic bacteria from wild and cultivated soybean varieties. Journal of Biol Fertil Soils. 2007; 44:155–162.
3. Sivakumar K. Centre of advanced study in marine biology. Annamalai University: 197-204.
4. Debananda S, Ningthoujam, Suchitra S, and Salam N. Screening of Actinomycete Isolates from Niche Habitats in Manipur for Antibiotic Activity. American Journal of Biochem and Biotech . 2009; 5: 221-225.
5. Kavya D, Solomon S, Nagalakshmi D. Isolation and screening of Streptomyces sp. from coringa mangrove soils for enzyme production and antimicrobial activity. International journal of Pharmaceutical, chemical and biological sciences, 2012; 2:110-116.
6. Dharna S, Anjali S. Isolation and screening of actinomycetes from mangrove soil for enzyme production and antimicrobial activity. International Journal of Research and Scientific Innovation, 2016; 2321–2705.
7. Silambarasan S, Praveen kumar E, Murugan T, Saravanan D, Balagurunathan R. Antibacterial and antifungal activities of actinobacteria isolated from Rathnagiri hills. Journal of Applied Pharmaceutical Science; 2012; 2: 099-103.
8. Kaur S, Kaur HP, Kaur G. Isolation and characterization of antibiotic producing actinomycetes from agriculture soil. World Journal Of Pharmacy And Pharmaceutical Sciences; 5: 1109-1117.
9. Gebreselema G, Feleke M, Samuel S, Nagappan R. Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia. Asian Pac J Trop Biomed . 2013; 3): 426-435.
10. Pradhan S, Mishra BB, Rout S. Screening of novel actinomycetes from near lake shore sediment of the chilika lake, Odisha, India.International Journal of Current Micobiology and Applied Science, 2015; 4:66-82.
11. Lisboa MP, Bonatto D, Bizani D, Henriques J and Brandelli AP. Characterization of a Bacteriocin- like Substance produced by Bacillus amyloliquefaciens isolated from the Brazilian Atlantic Forest. Journal of International Microbiology, 2006; 9:111-118.
12. Hung PQ, Kumar SM, Govindsamy V and Annapurna K. Isolation and characterization of Endophytic bacteria from wild and cultivated soybean varieties. Journal of Biol Fertil Soils. 2007; 44: 155–162.
13. Tautorus T E and Townsley P M. Analysis of an Effective Antibiotic (Chaetomacin) Isolated from a Thermophilic Bacillus sp. Against Olive Green Mold. Applied and environmental microbiology; 1984:775-779.
14. Augustine SK, Bbhavsar SP and Kapadnis BP. A non-polyene antifungal antibiotic from Streptomyces albidoflavus PU 23. Journal of Biosciences. 2005; 30: 201–211.
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How to Cite
Abbas, S., Gautam, G., & Gautam, P. K. (2019). Isolation and Screening of Actinomycetes producing Antibacterial compounds from different river sediments. Journal of Drug Delivery and Therapeutics, 9(3-s), 119-123. https://doi.org/10.22270/jddt.v9i3-s.2807