EFFECT OF DICLOFENAC SODIUM ON GLYCOGEN CONTENT OF ZEBRA FISH DANIO RERIO
Number of pharmaceuticals are commonly used now-a-days for the treatment of various ailments. Among them, Diclofenac sodium is used to relief pain and joint stiffness but the residual part of it exerts various side effects. It has been shown that the residue of diclofenac reaches in considerably high amounts in aquatic environment which causes toxicity to aquatic animals especially in fishes. Therefore, the aim of this experimental work is to observe the effect of diclofenac sodium in the glycogen content of zebra fish in three different tissues i.e. liver, gills and muscles. LC50 at 24 hours exposure of diclofenac sodium estimated by direct interpolation method was found to be 26.25mg/l. To observe the effect of diclofenac sodium on glycogen content, the fishes were divided into 4 groups. Group 1 served as untreated control. Group 2 was treated with 24hrs LC50 of diclofenac. Group 3 & 4 received 1/5th conc. of 24hrs LC50 for 7 and 15 days respectively. Fishes were sacrificed after lethal and sub lethal exposure. Liver, gills and muscles were separately analysed to study the glycogen content by Anthrone reagent test. It was observed that glycogen content drastically decreased within 24 hours of diclofenac treatment & then gradually increased in 7 & 15 days treatment. Thus, this study indicates that level of glycogen can greatly be altered in response to the toxic effects caused by diclofenac in zebra fish.
KEY WORDS: Diclofenac, Zebra fish, glycogen, LC50.
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