Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Metformin HCl and Repaglinide in Pharmaceutical Formulation

  • Sukanya Kharbade Priyadarshini J. L. College of Pharmacy, Electronic Building, Electronic Zone, Hingna Road, Nagpur-440016
  • Alpana Asnani Priyadarshini J. L. College of Pharmacy, Electronic Building, Electronic Zone, Hingna Road, Nagpur-440016
  • Kumar Pratyush Priyadarshini J. L. College of Pharmacy, Electronic Building, Electronic Zone, Hingna Road, Nagpur-440016

Abstract

Objective: A new, simple, rapid, accurate and economical method have been developed for the simultaneous estimation of Metformin HCl and Repaglinide in formulation.


Method: The absorbances of both the drugs were determined at 238 nm and at 294 nm. The linearity was observed in the concentration range of 2-100 µg/ml and 1-35 µg/ml for Metformin HCl and Repaglinide respectively. The method was validated as per ICH guidelines. 


Result: The recovery of Metformin and Repaglinide was found in the range of 98.24 ± 0.325 to 100.25 ± 0.756.


Conclusion: The proposed method was accurate, reproducible and economical and can be used successfully for quantitative estimation of Metformin HCl and Repaglinide in bulk and tablet dosage form.


Keyword:- Metformin, Repaglinide, UV- spectrophotometric method, simultaneous estimation

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Author Biographies

Sukanya Kharbade, Priyadarshini J. L. College of Pharmacy, Electronic Building, Electronic Zone, Hingna Road, Nagpur-440016

Priyadarshini J. L. College of Pharmacy, Electronic Building, Electronic Zone, Hingna Road, Nagpur-440016

Alpana Asnani, Priyadarshini J. L. College of Pharmacy, Electronic Building, Electronic Zone, Hingna Road, Nagpur-440016

Priyadarshini J. L. College of Pharmacy, Electronic Building, Electronic Zone, Hingna Road, Nagpur-440016

Kumar Pratyush, Priyadarshini J. L. College of Pharmacy, Electronic Building, Electronic Zone, Hingna Road, Nagpur-440016

Priyadarshini J. L. College of Pharmacy, Electronic Building, Electronic Zone, Hingna Road, Nagpur-440016

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How to Cite
Kharbade, S., Asnani, A., & Pratyush, K. (2019). Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Metformin HCl and Repaglinide in Pharmaceutical Formulation. Journal of Drug Delivery and Therapeutics, 9(3), 344-347. https://doi.org/10.22270/jddt.v9i3.2676