Assessment of Anthelmintic Activity of Ethanolic Extract of Musa sapientum Stem: An In-Vitro Approach

  • G. Manish Adithya
  • K. Pravalika
  • K. Bhavani
  • K. Thanuja
  • Bakshi Vasudha
  • Boggula Narender
  • Sayeed Mohammed


Many medicinal plants claimed to possess anthelmintic activity. Musa sapientum belonging to the Musaceae family is a highly valued plant, distributed in many countries of the tropical and sub tropical. Its common names includes banana. Some standard drugs such as piperazine citrate, albendazole, mebendazole, thiabendazole possess some side effects such as nausea, vomiting, stomach and abdominal pain, headache, dizziness, and temporary hair loss etc. But the herbal drug shows fewer side effects. Musa sapientum L. (Musaceae) are mainly grown in the tropical and subtropical countries and are widely used for its nutritional values all over the world. Preliminary phytochemical investigation includes the presence of alkaloids, carbohydrates, glycosides, flavonoids, steroids and tannins. Indian adult earthworms (Pheretima posthuma) were used to study anthelmintic activity. The activity was checked in ethanolic extract with three different concentrations (100, 200, 300mg/ml) and compared with the standard drug Albendazole (10mg/ml) and control as distilled water. The result was expressed in the terms of paralysis time and death time of worms. Ethanolic extract of Musa sapientum stem shows anthelmintic activity in dose dependent manner and maximum efficacy is seen at 300mg/ml concentration. Hence it was concluded that ethanolic extract of Musa sapientum stem have anthelmintic property.

Keywords: Musa sapientum, earthworms, anthelmintic activity, albendazole, paralysis, Pheretima posthuma.


Download data is not yet available.


1. Hotez PJ, Brindley PJ, Bethony JM, King CH, Pearce EJ, Jacobson J. Helminth infections: the great neglected tropical diseases. The Journal of Clinical Investigation 2008; 118:1311-21.
2. Mulla WA, Thorat VS, Patil RV, Burade KB. Anthelmintic activity of leaves of Alocasia indica Linn. International Journal of PharmTech Research 2010; 2(1):26-30.
3. Clewes CAN, Shaw C. Parasites. British Medical Bulletin 2000; 56(1):193-208.
4. Barnabas BB, Mann A, Ogunrinola TS, Anyanwu PE. Screening for Anthelminthic activities from extracts of Zanthoxylum zanthoxyloides, Neocaryamacrophylla and Celosia laxa against ascaris infection in rabbits. International Journal of Applied Research in Natural Products 2010; 3(4):1-4.
5. Mohamed AAER, Ismail MAN, Mohamed AO, Moustafa FM. Cytotoxic effects of albendazole, antiparasitic drug, on the liver of the rat: Subchronic study. Egyptian journal of Biology 1999; 1:16-29.
6. Dwivedi A, Dwivedi S, Sitoke AK, Patel R, Jhade D. Anthelmintic activity of a Polyherbal preparation. Ethnobotanical Leaflets 2009; 13:259-62.
7. Aremu OA, Fawole JC, Chukwujekwu ME, Light JF, Finnie J, Staden V. In vitro antimicrobial, anthelmintic and cyclo-oxygenase inhibitory activities and phytochemical analysis of Leucosidea sericea. Journal of ethnopharmacology 2010; 131:22-7.
8. Velan SS, Suresh A, Suresh V, Kumar SN, Bright AJ, Karthikeyan P. In vitro anthelmintic activity of Samanea saman (Merr) bark. Journal of Pharmacy Research 2010; 3(12):2977-8.
9. Devi K, Indumathy S, Rathinambal V, Uma S, Kavimani S, Balu V. Anthelminthic Activity of AstaChurna. International Journal of Health Research 2009; 2(1):101-3.
10. Aswar M, Aswar U, Watkar B, Vyas M, Wagh A, Gujar KN. Anthelmintic activity of Ficus benghalensis. International Journal of Green Pharmacy 2008; 2:170-2.
11. Jabbar A, Raza MA, Iqbal Z, Khan MN. An inventory of the ethnobotanicals used as anthelmintics in the southern Punjab (Pakistan). Journal of Ethnopharmacology 2006; 108:152-4.
12. Iqbal Z, Lateef M, Ashraf M, Jabbar A. Anthelmintic activity of Artemisia brevifolia in sheep. Journal of Ethnopharmacology 2004; 93:265-8.
13. Ahmad I, Beg AZ. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. J Ethnopharmacol 2001; 74:113-23.
14. Mokbel MS, Hashinaga F. Antibacterial and Antioxidant Activities of Banana (Musa, AAA cv. Cavendish) Fruits Peel. Am J Biochem Biotechnol. 2005; 1(3):125-31.
15. Narender Boggula, Swetha Reddy Naram Reddy, Teja Sri Alla, Azmath Farhana, Jainendra Battineni, Vasudha Bakshi. Phytochemical evaluation and in vitro anti bacterial activity of dried seeds of Abrus precatorius. International journal of pharmaceutical sciences review and research, 2017, 44(1):101-107.
16. Alisi CS, Nwanyanwu CE, Akujobi CO, Ibegbulem CO. Inhibition of dehydrogenase activity in pathogenic bacteria isolates by aqueous extracts of Musa paradisiaca (var. sapientum). Afr J Biotechnol. 2008; 7(12):1821-5.
17. Narender Boggula, Himabindu Peddapalli. Phytochemical analysis and evaluation of in vitro anti oxidant activity of Punica granatum leaves. International Journal of Pharmacognosy and Phytochemical Research 2017, 9(8); 1110-1118.
18. Mohammed Sayeed, Vasudha Bakshi, Narender Boggula. Phytochemical and anthelmintic screening of Moringa oleifera stem ethanolic extract. Indo American Journal of Pharmaceutical Research 2017; 7(12):981-91.
19. Swetha Naram Reddy, Susheel Rao Yeedulapally, Geetha Vani Palusam, Nikhil Pathi, Mani Swaroop Goud Gunala, Vasudha Bakshi, Narender Boggula. Assessment of anthelmintic activity and phytochemical analysis of Psidium guajava leaves – An in-vitro design. Journal of Pharmaceutical and Scientific Innovation. 2018; 7(3): 82-87.
0 Views | 5 Downloads
How to Cite
Manish Adithya, G., Pravalika, K., Bhavani, K., Thanuja, K., Vasudha, B., Narender, B., & Mohammed, S. (2019). Assessment of Anthelmintic Activity of Ethanolic Extract of Musa sapientum Stem: An In-Vitro Approach. Journal of Drug Delivery and Therapeutics, 9(3), 319-324.

Most read articles by the same author(s)