DETERMINATION OF CETIRIZINE IN HUMAN PLASMA AND ITS VALIDATION OF METHOD USING HPLC TECHNIQUE
Cetrizine determination in human plasma using HPLC technique
This reseach project is dedicated to analyze cetirizine in human plasma by using HPLC qualitatively and quantitatively, to make a reliable bioanalysis of this drug in human plasma with a goal to utilise it in pharmacokinetics studies. In this research project, cetirizine was extracted from plasma using liquid-liquid extraction by dichloromethane and ethyl acetate. The results in terms of accuracy and precision are compared. For both of the methods, amlodipine was used as the internal standard. Separation was carried out by Phenomenex C18 chromatography column. The mobile phase used here is 35:65, v/v of Acetonitrile to 0.3% triethylamine (TEA) buffer fixed at pH 3 by phosphoric acid, with flow rate of 1mL/min. The column temperature was set at 30 ᵒC. Various wavelength detector (VWD) was used, and detection was set at 237nm. The analysis was linear from 30ng/mL to 500ng/mL. Overall the recovery is more than 90% when using dichloromethane and more than 65% when using ethyl acetate.
Keywords: Bioanalysis, Method validation, Cetirizine, Human plasma, HPLC
2. De Jager, a. D., Hundt, H. K. L., Swart, K. J., Hundt, A. F., & Els, J. Extractionless and sensitive method for high-throughput quantitation of cetirizine in human plasma samples by liquid chromatography–tandem mass spectrometry. J Chromatogr B, 2002; 773(2):113–118. http://doi.org/10.1016/S1570-0232(02)00134-4
3. Kim, C.-K., Yeon, K. J., Ban, E., Hyun, M.-J., Kim, J.-K., Kim, M.-K., Park, J.-S. Narrow-bore high performance liquid chromatographic method for the determination of cetirizine in human plasma using column switching. J Pharm Biomed Ana, 2005; 37(3):603–9. http://doi.org/10.1016/j.jpba.2004.11.022
4. Dharuman, J., Vasudhevan, M., & Ajithlal, T. High performance liquid chromatographic method for the determination of cetirizine and ambroxol in human plasma and urine-A boxcar approach. J Chromatogr B Analyt Technol Biomed Life Sci, 2011; 879(25):2624–2631. http://doi.org/10.1016/j.jchromb.2011.07.024
5. Wichitnithad, W., Jithavech, P., Sanphanya, K., Vicheantawatchai, P., & Rojsitthisak, P. Determination of Levocetirizine in Human Plasma by LC–MS-MS: Validation and Application in a Pharmacokinetic Study. J Chromatogr Sci, 2015; 53(10):1663-1672. https://doi.org/10.1093/chromsci/bmv069
6. Eriksen, H., Houghton, R., Green, R., & Scarth, J. Determination of cetirizine in human plasma by liquid chromatography-tandem mass spectrometry. Chromatographia, 2002; (8):145–149. http://doi.org/10.1007/BF02493371.
7. Macek, J., Ptácek, P., & Klíma, J. Determination of cetirizine in human plasma by high-performance liquid chromatography. J Chromatogr B Biomed Sci Appl, 1999; 736(1–2):231–5. https://doi.org/10.1016/S0378-4347(99)00464-8
8. Zaater, M. F., Tahboub, Y. R., & Najib, N. M. RP-LC method for the determination of cetirizine in plasma. J Pharm Biomed Anal, 2000; 22(5):739–744. http://doi.org/10.1016/S0731-7085(99)00294-0.
9. Esteve-Romero, J., Albiol-Chiva, J., & Peris-Vicente, J. A review on development of analytical methods to determine monitorable drugs in plasma and urine by micellar liquid chromatography using direct injection. Anal. Chim. Acta, 2016; 926:1–16. http://doi.org/10.1016/j.aca.2016.04.026.
10. Pandey, S., Pandey, P., Tiwari, G., & Tiwari, R. Bioanalysis in drug discovery and development. Pharma Meth, 2010; 1(1):14–24. http://doi.org/10.4103/2229-4708.72223.
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