DETERMINATION OF CETIRIZINE IN HUMAN PLASMA AND ITS VALIDATION OF METHOD USING HPLC TECHNIQUE

Cetrizine determination in human plasma using HPLC technique

  • Siti Hazar Binti Zuhri Department of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia.
  • A.K. Azad Advanced drug delivery Laboratory, Faculty of Pharmacy, International Islamic University, Jalan Sultan Ahmad Shah
  • Abdur Rashid Mia Advanced Drug Delivery Laboratory, Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia.
  • ABM Helal Uddin Department of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia.
  • Mohamed Sufian Bin Mohd. Nawi Department of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia.

Abstract

This reseach project is dedicated to analyze cetirizine in human plasma by using HPLC qualitatively and quantitatively, to make a reliable bioanalysis of this drug in human plasma with a goal to utilise it in pharmacokinetics studies. In this research project, cetirizine was extracted from plasma using liquid-liquid extraction by dichloromethane and ethyl acetate. The results in terms of accuracy and precision are compared. For both of the methods, amlodipine was used as the internal standard. Separation was carried out by Phenomenex C18  chromatography column. The mobile phase used here is 35:65, v/v of Acetonitrile to 0.3% triethylamine (TEA) buffer fixed at pH 3 by phosphoric acid, with flow rate of 1mL/min. The column temperature was set at 30 ᵒC. Various wavelength detector (VWD) was used, and detection was set at 237nm. The analysis was linear from 30ng/mL to 500ng/mL. Overall the recovery is more than 90% when using dichloromethane and more than 65% when using ethyl acetate.


Keywords: Bioanalysis, Method validation, Cetirizine, Human plasma, HPLC     

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Author Biographies

Siti Hazar Binti Zuhri, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia.

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia.

A.K. Azad, Advanced drug delivery Laboratory, Faculty of Pharmacy, International Islamic University, Jalan Sultan Ahmad Shah

 Advanced Drug Delivery Laboratory, Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia.

Abdur Rashid Mia, Advanced Drug Delivery Laboratory, Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia.

 Advanced Drug Delivery Laboratory, Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia.

ABM Helal Uddin, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia.

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia.

Mohamed Sufian Bin Mohd. Nawi, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia.

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia.

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How to Cite
Binti Zuhri, S. H., Azad, A., Mia, A. R., Uddin, A. H., & Mohd. Nawi, M. S. B. (2019). DETERMINATION OF CETIRIZINE IN HUMAN PLASMA AND ITS VALIDATION OF METHOD USING HPLC TECHNIQUE. Journal of Drug Delivery and Therapeutics, 9(2-s), 243-247. https://doi.org/10.22270/jddt.v9i2-s.2650