Extraction, Isolation and Characterization Screening of Coccinia grandis
Herbal medicine is the oldest form of medicine known to mankind. It was the mainstay of many early civilization and still the most widely practiced form of medicine in the world today. Medicinal plants produce a diverse range of bioactive molecules, making them rich sources of different types of medicines. Coccinia grandis whole plant part is used in Siddha, Ayurveda, Unani, traditional and ethano medicinal system. The present study is focus on the phytochemical analysis of ethanolic extract of Coccinia grandis aerial parts. Qualitative and quantitative phytochemical analysis of this species extract confirms the presence of various phytochemicals like alkaloids, glycosides, phenolic compounds and steroids. Purification of isolated compounds has been done and characterization of isolated compounds performed through spectral techniques like IR, NMR, UV, Mass spectrum. UV spectra have shown maximum absorption due to substitution of functional group in compound A. The IR spectrum of compound A showed absorption bands characteristics of flavanol indicating the presence of hydroxyl and tinsoporin functionalities at 3449, 1375 and 1079 cm-1 respectively, indication of double bond were represented by 1689 cm-1. Peak at 1375 cm-1 represented C-H bending whereas NMR was also characteristic of phytotaxanol, exhibiting the hydroxyl proton signal at δ 5.21as a multiplet, which helped us to characterize this compound as taxaterone, tinsoporin A and B respectively
Keywords- Coccinia grandis, Phytochemical screening, Isolation, Mass spectrum
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