Extraction, Isolation and Characterization Screening of Coccinia grandis

  • L G P Ram Bindurani Faculty of Pharmacy, Bhagwant University, Ajmer Rajasthan, INDIA
  • Anoop Singh Faculty of Pharmacy, Bhagwant University, Ajmer Rajasthan, INDIA


Herbal medicine is the oldest form of medicine known to mankind. It was the mainstay of many early civilization and still the most widely practiced form of medicine in the world today. Medicinal plants produce a diverse range of bioactive molecules, making them rich sources of different types of medicines. Coccinia grandis whole plant part is used in Siddha, Ayurveda, Unani, traditional and ethano medicinal system. The present study is focus on the phytochemical analysis of ethanolic extract of Coccinia grandis aerial parts. Qualitative and quantitative phytochemical analysis of this species extract confirms the presence of various phytochemicals like alkaloids, glycosides, phenolic compounds and steroids. Purification of isolated compounds has been done and characterization of isolated compounds performed through spectral techniques like IR, NMR, UV, Mass spectrum. UV spectra have shown maximum absorption due to substitution of functional group in compound A. The IR spectrum of compound A showed absorption bands characteristics of flavanol indicating the presence of hydroxyl and tinsoporin functionalities at 3449, 1375 and 1079 cm-1 respectively, indication of double bond were represented by 1689 cm-1. Peak at 1375 cm-1 represented C-H bending whereas NMR was also characteristic of phytotaxanol, exhibiting the hydroxyl proton signal at δ 5.21as a multiplet, which helped us to characterize this compound as taxaterone, tinsoporin A and B respectively

Keywords- Coccinia grandis, Phytochemical screening, Isolation, Mass spectrum


Download data is not yet available.

Author Biographies

L G P Ram Bindurani, Faculty of Pharmacy, Bhagwant University, Ajmer Rajasthan, INDIA

Faculty of Pharmacy, Bhagwant University, Ajmer Rajasthan, INDIA

Anoop Singh, Faculty of Pharmacy, Bhagwant University, Ajmer Rajasthan, INDIA

Faculty of Pharmacy, Bhagwant University, Ajmer Rajasthan, INDIA


1. Pekamwar SS, Kalyankar TM., and Kokate SS. Pharmacological Activities of Coccinia Grandis: Review Journal of Applied Pharmaceutical Science Vol. 3 (05):114-119
2. Ajmal AM, Pandey AK. Systematic Studies on the Family Cucurbitaceae of Eastern Bihar, India. Cucurbit Genetics Cooperative Report. 2005-2006; 28-29, 66-69.
3. Harshitha Y, Prasanthi NL. And Ramarao N, Coccinia grandis:A pharmaceutical review International Journal of Pharma And Chemical Research, 2018; 4(2):117-124
4. Bhattacharya B et al., In-vitro evaluation of antifungal and Antibacterial activities of the plant Coccinia grandis (L.) Voigt. (Family- Cucurbitaceae). Journal of Phytology. 2010; 2(11):52-57.
5. Bhattacharya B et al., In-vivo and in-vitro anticancer activity of Coccinia grandis (L.) Voigt. (Family: Cucurbitaceae) on Swiss albino mice. Journal of Pharmacy Research. 2011; 4(3):567-569.
6. Tamilselvan N, Thirumalai T, Elumalai EK, Balaji R, David E, Pharmacognosy of Coccinia grandis: a review, Asian Pacific Journal of Tropical Biomedicine 2011; 1(2):300-302
7. Khandelwal KR, Practical Pharmacognosy: Techniques & Experiments, 3rd Edition, Nirali Prakashan, Mumbai, 2002, 35-40
8. Stahl E. (ed.), Thin-Layer Chromatography, Springer-Verlag Berlin Heidelberg 1969, 421-471
9. Aggarwal AS. et al. Analgesic and antipyretic activity of methanolic extract of Coccinia grandis L. Leaves in experimental animals. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2011; 2:175-182.
10. Lather AM, Bole NS Vedamurthy AB, In vitro antioxidant and anti-inflammatory activity of Coccinia grandis. International Journal of Pharmacy and Pharmaceutical Sciences. 2012; 4:239-242.
11. Behera SK, Dash V. Some Indian vegetable used as an anticancer agent. International journal of advanced pharmaceutical and biological sciences. 2012; 2:250-264.
12. Bhuiyan MN et al., Mutagenic effect of Coccinia cordifolia leaf extract on n.crassa fu gus. Bangladesh journal of science and research. 2009; 44:215-220.
13. Bulbul IJ, Nathan L., Haque M. Antibacterial Cytotoxic and antioxidant activity of chloroform, n-hexane and ethyl acetate extract of plant Coccinia cordifolia. Agriculture and Biology journal of North America. 2011; 2(4):713-719.
14. Deshpande SV., In-vitro antioxidant study of petroleum ether chloroform and ethyl acetate fractions of coccinia grandis stems extract. International journal of chemical sciences. 2011; 9 (1):80-86.
15. Deshpande SV, Patil MJ., Parmar KK., Daswadkar SC. And Khodade RB. A study of antioxidant activity of fruit extract of Coccinia grandis Lvoight. International Journal of Drug Research and Technology. 2011; 1 (1): 69-72.
16. Deokate UA. and Khadabadi SS.. Pharmacology and photochemistry of Coccinia indica. Journal of Pharmacognosy and Phytotherapy. 2011; 3(11): 155-159.
17. Deshpande SV., Patil MJ, Daswadkar SC., Suralkar U, Agarwal AA study on antii flammatory activity of the leaf and stem extract of Coccinia grandis. vioght. International Journal of Applied Biology and Pharmaceutical Technology. 2011; 2:247-250.
18. Dewanjee S et al. In-Vitro Evaluation of Antimicrobial Activity of Crude Extract from Plants Diospyros peregrina, Coccinia grandis and Swietenia macrophylla. Tropical Jou nal of Pharmaceutical Research. 2007; 6(3):773-778.
19. Doss A. and Dhanabalan R. Anti-hyperglycemic and Insulin Release Effects of Coccinia grandis (L.) Voigt Leaves in Normal and Alloxan Diabetic Rats. Ethno botanical Leaflets. 2008; 12:1172-75.
20. Krishnkumari S., Bhuvaneshwari P., Rajeswari P. The ameliorative potential of Coccinia grandis of serum and liver marker enzymes and lipid profile in streptozotocin I duced
21. Manjula S., Ragavan B. Hypoglycemic and Hypolipidemic effect of Coccinia indica Wight &Arn in alloxan induced diabetic rats. 2007; 27(2):34-7.
22. Kavitha A, Krishna Reddy B, Niharika J, Venkata Rao Vutla, Narendra Babu A Evaluation of antibacterial activity of Coccinia grandis leafs and stem extracts The Pharma Innovation Journal 2015; 4(4):01-03
294 Views | 234 Downloads
How to Cite
Ram Bindurani LGP, Singh A. Extraction, Isolation and Characterization Screening of Coccinia grandis. JDDT [Internet]. 15May2019 [cited 19Jan.2021];9(3):238-45. Available from: http://jddtonline.info/index.php/jddt/article/view/2643