Determination of Antibody Titration between Clinical and Community-Based Patients for O, H, AH and BH Antigens in Salmonella Samples

  • Nisar Ahmad Wani
  • Jaianand Kannaiyan
  • Balaji Paulraj
  • Veeramanikandan Veeramani

Abstract

The aim of the study is to determine the baseline antibody titer of Salmonella bacteria in different positive samples with a view to establishing the significant titer for Widal agglutination test in Kashmir. The Widal test was performed on each serum. The slide agglutination test was first done and then positive samples were further subjected to tube agglutination for quantitative titration. The most commonly utilized diagnostic test for enteric fever is a Widal test, which detects agglutinating antibodies against the O, H, AH and BH antigens of S. typhi. The results of the Widal test showed that out of 413 samples 219 were positive for Antigen O, 165 were positive for Antigen H, 17 were positive for Antigen AH and 12 were positive for Antigen BH. The highest percentage cases were with Antigen-O whereas the lowest percentage was found in Antigen-BH. The difference between clinical and community-based patients have been studied.


Keywords: Aetiology, Antigen, Enteric fever, Morbidity, Salmonella typhi, Widal test.

Downloads

Download data is not yet available.

References

1. Wong VK, Baker S, Pickard DJ, et al. Phylogeographical analysis of the dominant multidrug-resistant H58 clade of Salmonella typhi identifies inter- and intracontinental transmission events. Nat Genet 2015; 47:632–9.
2. Keddy KH, Sooka A, Letsoalo ME, et al. Sensitivity and specificity of typhoid fever rapid antibody tests for laboratory diagnosis at two sub-Saharan African sites. Bull World Health Organ 2011; 89:640–7.
3. Andrews JR, Ryan ET. Diagnostics for invasive Salmonella infections: current challenges and future directions. Vaccine 2015; 33:C8–15.
4. Date KA, Bentsi-Enchill A, Marks F, Fox K. Typhoid fever vaccination strategies. Vaccine 2015; 33:C55–61.
5. Bhunia AK., Foodborne microbial pathogens: Mechanisms and pathogenesis. United States of America: Springer Science + Business Media, LLC. 2008.
6. Yousef AE., Carlstrom C., Salmonella. In Yousef, A. E. and Carstrom, C. (Eds.). Food microbiology: A laboratory manual. 2003; 167-205.
7. Montville TJ., Matthews KR., Food microbiology: an introduction. 2nd ed. Washington, USA: ASM Press.Mweu E, English M. 2008.
8. Hosoglu S., Celen M., Geyik MF., Risk factors for typhoid fever among adult patients in Diyarbakir, Turkey. Epidemiol Infect. 2006; 134:612-6.
9. Ram PK., Naheed A., Brooks WA et al. Risk factors for typhoid fever in a slum in Dhaka, Bangladesh. Epidemiol Infect. 2007; 135:458-65.
10. Gerald L., Mandel., John E., Bennett., Rapheal D., Principles and Practice of Infectious Diseases, 5th edition, Volume-2, Churchill Living Stone. 2000; (210):2344-2363.
11. Wilson JC., A treatise on the continued fevers, Wood, New York. 1881.
12. Corkill G., Rapley R., The Manipulation of Nucleic Acids: Basic Tools & Techniques in Molecular Bio Methods Handbook Second Edition. Ed: Walker J M, Rapley R (NJ: Humana Press). 2008.
13. Drancourt M., Bollet C., Carlioz A., Martelin R., Gayral JP., Raoult D., 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. J Clin Microbiol. 2000; 38:3623–3630.
14. Mignard S., Flandrois JP., 16S rRNA sequencing in routine bacterial identification: a 30-month experiment. J Microbiol Methods. 2006; 67:574–581.
15. Fox GE., Wisotzkey 1D., Jurtshuk, P., (1992). How close is close: 16s rRNA sequence identity may not be sufficient to guarantee species identity. Int J Syst Bacteriol. 1992; 42, 166-170.
16. Nisar AW., Veeramanikandan V., Jaianand K., Balaji P., Evaluation of clinical profile and response of various antibiotics in Typhoid fever. International Journal of Medical Science and Diagnosis Research. 2018; 2(4): 166-171.
17. Veeramanikandan V., Nisar AW., Jaianand K., Balaji P., Prevalence and different laboratory diagnosis methods for the determination of typhoid fever in Kashmir region of Jammu and Kashmir, India. Journal of Medical science and Clinical Research. 2018; 06(08): 861-866.
Statistics
32 Views | 74 Downloads
How to Cite
Wani, N. A., Kannaiyan, J., Paulraj, B., & Veeramani, V. (2019). Determination of Antibody Titration between Clinical and Community-Based Patients for O, H, AH and BH Antigens in Salmonella Samples. Journal of Drug Delivery and Therapeutics, 9(3), 194-199. https://doi.org/10.22270/jddt.v9i3.2640