Identification of potential thermo stable enzyme producers and antibacterial activity of hot spring bacteria against multi drug resistant bacterial strains
In this study the sediment samples of Rajapur-unhale and Sangameshwar- Aravali hot springs were collected and aimed to screen the antimicrobial resistance of the isolates against MDR strains and screening of potential enzyme producing strains from hot springs. Seventy seven (77) isolates were obtained from Rajapur-Unhale sediment and in Sangameshwar-Aravali sediment forty two (42) isolates were obtained. Out of 119 isolates five isolates (4.20 %) were positive for amylase activity, twenty one isolates (17.64 %) were positive for protease, four isolates (3.36 %) were positive for lipase enzyme production, eight isolates (6.72 %) were positive for keratinase enzyme and four isolates (3.36%) were positive for urease enzyme. In primary screening, 19 isolates (15.96 %) exhibited antimicrobial activity against Klebsiella pneumonia ATCC 43816T, whereas the isolates were not shown any kind of activity against Pseudomonas aeruginosa – MTCC 2453T and Staphylococcus aureus – ATCC 43300T. Nineteen isolates exhibited antimicrobial activity showing promising activity against test organisms out of which sixteen isolates (13.44 %) were positive for Raajapur-Unhale hot spring. Whereas, in Sangameshwar-Aravali hot spring only three isolates (2.52 %) showed positive zone of inhibition against Klebsiella pneumonia ATCC 43816T. The Fifty five isolates obtained from enzyme screening and antimicrobial screening were identified through 16S rRNA gene sequencing, the sequences were assembled and identified through Ez-Taxon server by blast search analysis of the sequences in fasta format.
Keywords: Enzyme screening, Antimicrobial activity, Multi Drug Resistant, 16S rRNA gene sequencing
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