Development and Validation of RP-HPLC Method for Desoximetasone in Bulk and Cream Formulation

  • Ashish Gorle Department of Pharmaceutics, R C Patel Institute of Pharmaceutical Education and Research Shirpur, Dhule, Maharashtra 425405, India
  • Jayashri Mahajan Department of Pharmaceutics, R C Patel Institute of Pharmaceutical Education and Research Shirpur, Dhule, Maharashtra 425405, India
  • Prathamesh Bhave Department of Pharmaceutics, R C Patel Institute of Pharmaceutical Education and Research Shirpur, Dhule, Maharashtra 425405, India

Abstract

Desoximetasone chemically is 9-fluoro-IIβ21-dihydroxy-I6a-methylpregna-I.4-diene-3.The precise mechanism of the anti-inflammatory activity of topical steroids in the treatment of steroid-responsive dermatoses, in general, is uncertain. So, in present investigation chromatographic methods were developing use RP-HPLC for estimation of Desoximetasone in bulk and in cream formulation and method validation according to ICH guidelines. The main objective of this study was to develop a simple and reproducible method for desoximetasone by Reverse Phase High Performance Liquid Chromatography (RP-HPLC). In this work the desoximetasone separation was carried out by using C18 cosmosile column (250mmx4.6mm particle size 5µm). By using 0.1% orthrophosphoric acid pH adjusted up to 3 at uv detection of 240nm.The mobile phase was used at various ratio for gradient elution the ratio of mobile phase was 20:80 v/v. Methanol and water used for mobile phase and flow rate was being set at 1mL/min. The linearity of proposed method was found in range of r =0.9989. Statistically validation parameters such as linearity, accuracy, precision, LOD and LOQ were checked.


Keywords: Desoximetasone, RP-HPLC, Method validation.

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Author Biographies

Ashish Gorle, Department of Pharmaceutics, R C Patel Institute of Pharmaceutical Education and Research Shirpur, Dhule, Maharashtra 425405, India

Department of Pharmaceutics, R C Patel Institute of Pharmaceutical Education and Research Shirpur, Dhule, Maharashtra 425405, India

Jayashri Mahajan, Department of Pharmaceutics, R C Patel Institute of Pharmaceutical Education and Research Shirpur, Dhule, Maharashtra 425405, India

Department of Pharmaceutics, R C Patel Institute of Pharmaceutical Education and Research Shirpur, Dhule, Maharashtra 425405, India

Prathamesh Bhave, Department of Pharmaceutics, R C Patel Institute of Pharmaceutical Education and Research Shirpur, Dhule, Maharashtra 425405, India

Department of Pharmaceutics, R C Patel Institute of Pharmaceutical Education and Research Shirpur, Dhule, Maharashtra 425405, India

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How to Cite
Gorle, A., Mahajan, J., & Bhave, P. (2019). Development and Validation of RP-HPLC Method for Desoximetasone in Bulk and Cream Formulation. Journal of Drug Delivery and Therapeutics, 9(3), 154-159. https://doi.org/10.22270/jddt.v9i3.2630