Characterization and phylogenetic analysis of alkaline α-amylase producing Brevibacillus laterosporus from mountain climatic zone of India
α-amylases (EC126.96.36.199) are glycoside hydrolases that breakdown complex starch and maltodextrins into glucose and maltose by acting upon 1,4-glycosidiclinkages. Several amylases have been isolated and purified from members of Bacillus community, which find extensive application in starch processing, textile and pharmaceutical industry. Keeping this in mind we isolated α-amylase producing gram positive bacterium from soils collected from mountain climatic zone of India and identified it as Brevibacillus laterosporus. We further studied the effect of temperature and pH on the amylase activity of this strain and found a very stable activity at alkaline pH of 10 and temperature of 45 ºC. To our knowledge this a first report on characterization and evolutionary analysis of alkaline α-amylase producing Brevibacillus laterosporus isolated from unexplored sites of mountain climatic zone of India.
Keywords: Climatic zone, Brevibacillus, Amylase, 16S rRNA gene sequencing, Phylogenetic analysis
2. Peel MC, Finlayson BL, McMahon TA, Updated world map of the Köppen-Geiger climate classification, Hydrology and earth system sciences discussions, 2007; 4(2):439-73.
3. Kheyrodin H and Ghazvinian K, Effect of Climate Change on Soil Global Microorganism, Journal of Biological Chemistry, 2014; 2:310-319.
4. Classen AT, Sundqvist MK, Henning JA, Newman GS, Moore JAM, Cregger MA, Moorhead LC, Patterson CM, Direct and indirect effects of climate change on soil microbial and soil microbial plant interactions: What lies ahead? Ecosphere, 2015; 8:130.
5. Drenovsky RE, Steenwerth KL, Jackson LE, Scow KM, Land use and climatic factors structure regional patterns in soil microbial communities, Global Ecology and Biogeography, 2010; 1:27-39.
6. Shida O, Takagi H, Kadowaki K, Komagata K, Proposal for Two New Genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov., International Journal of Systematic and Evolutionary Microbiology, 1996; 46(4):939-46.
7. Available at http://www.bacterio.net/bacillus.html [Accessed 30 March 2019]
8. Available at http://www.bacterio.net/brevibacillus.html [Accessed 31 March 2019]
9. Filippidou S, Wunderlin T, Junier T, Jeanneret N, Dorador C, Molina V, Johnson DR, Junier P, A combination of extreme environmental conditions favor the prevalence of endospore-forming firmicutes, Frontiers in microbiology, 2016; 7:1707.
10. Nicholson WL, Munakata N, Horneck G, Melosh HJ, Setlow P, Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments, Microbiology Molecular Biology Reviews, 2000; 64(3):548-72.
11. Suneeva SC, Prasanth R, Rajesh NG, Viswanathan P, Transformation of Brevibacillus, a soil microbe to an uropathogen with hemagglutination trait, World Journal of Microbiology and Biotechnology, 2014; 30(6):1837-44.
12. Ruiu L, Brevibacillus laterosporus, a pathogen of invertebrates and a broad-spectrum antimicrobial species, Insects, 2013; 4(3):476-92.
13. Mahdhi A, Kamoun F, Messina C, Bakhrouf A, Probiotic properties of Brevibacillus brevis and its influence on sea bass (Dicentrarchus labrax) larval rearing, African Journal of Microbiology Research, 2012; 6(35):6487-95.
14. Panda AK, Bisht SS, DeMondal S, Kumar NS, Gurusubramanian G, Panigrahi AK, Brevibacillus as a biological tool: a short review, Antonie Van Leeuwenhoek, 2014; 105(4):623-39.
15. Hill R, The chemistry of life: eight lectures on the history of biochemistry, CUP Archive; 1970.
16. Souza PM, Application of microbial α-amylase in industry-A review, Brazilian journal of microbiology, 2010; 41(4):850-61.
17. Sundarram A, Murthy TP, α-amylase production and applications: a review, Journal of Applied & Environmental Microbiology, 2014; 2(4):166-75.
18. Nair GR, Raja SS, Climate and Soil Properties Influence Species Diversity of Soil Bacillus Community in India, Microbiology Insights, 2018; 11:1178636118810366.
19. Khan N, Vidyarthi A, Nadeem S, Negi S, Nair G, Agrewala JN, Alteration in the Gut microbiota Provokes Susceptibility to Tuberculosis, Frontiers in Immunology, 2016; 7:529.
20. Mishra S, Behera N, Amylase activity of a starch degrading bacteria isolated from soil receiving kitchen wastes, African Journal of Biotechnology, 2008; 7(18).
21. Xiao Z, Storms R, Tsang A, A quantitative starch? Iodine method for measuring alpha-amylase and glucoamylase activities, Analytical biochemistry, 2006; 351(1):146-8.
22. Pandey KK, Mayilraj S, Chakrabarti T. Pseudomonas indica sp. nov., a novel butane–utilizing species. Int J Syst Evol Microbiol, 2002; 52:1559–1567.
23. Nair RG, Kaur G, Khatri I, Singh NK, Maurya SK, Subramanian S, Behera A, Dahiya D, Agrewala JN, Mayilraj S. Genome Mining and Comparative Genomic Analysis of Five Coagulase-Negative Staphylococci (CNS) Isolated from Human Colon and Gall Bladder. J Data Mining Genomics Proteomics, 2016; 7:192.
24. Kumar S, Stecher G, Tamura K, MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets, Molecular Biology and Evolution, 2016; 33:1870-1874.
25. Felsenstein J, Confidence limits on phylogenies: an approach using the bootstrap, Evolution, 1985; 39:783-791.
26. Patel J, Meena RK, Sao S, Dubey SK, Shukla P, Amylase degrading bacteria from soil and their RAPD profiling. International journal of Current Microbiology and Applied Sciences. 2014; 3(7):55-63.
27. Amoozegar MA, Malekzadeh F, Malik KA, Production of amylase by newly isolated moderate halophile, Halobacillus sp. strain MA-2, Journal of microbiological methods, 2003; 52(3):353-9.
28. Yohandini H, Isolation and phylogenetic analysis of thermophile community within Tanjung Sakti hot spring, South Sumatera, Indonesia, HAYATI Journal of Biosciences, 2015; 22(3):143-8.
29. Bisht SS, Panda AK, Biochemical characterization and 16S rRNA sequencing of few lipase-producing thermophilic bacteria from Taptapani hot water spring, Orissa, India, Biotechnology research international, 2011; 2011.
30. Nwokoro O, Anthonia O, Studies on the production of alkaline?-amylase from Bacillus subtilis CB-18, Acta Scientiarum Polonorum Technologia Alimentaria, 2015; 14(1):71-5.
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